POLR1D Polyclonal Antibody for FACS, IHC, Western blot
Synonyms:
AC19, POLR1C, RPA16, RPA9, RPAC2, RPC16, RPO1-3, TCS2, DNA-directed RNA polymerase I subunit D, DNA-directed RNA polymerases I and III subunit RPAC2, RNA polymerases I and III subunit AC2
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Form:
100 ug of antibody purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS and supplied in PBS with 0.09% (W/V) sodium azide.
Applications
Dilution *
Western Blot (WB)
1:50-100
Immunohistochemistry (IHC)
1:50-100
Flow Cytometry (FACS)
1:10-50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-26114 targets POLR1D in FACS, IHC, and WB applications and shows reactivity with Human samples.
The PA5-26114 immunogen is synthetic peptide from the C-terminal region of human POLR1D conjugated to KLH.
General Information
Product Images
POLR1D Antibody (PA5-26114) in FACS
Flow Cytometry with anti-POLR1D Polyclonal Antibody (PA5-26114)
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
POLR1D Antibody (PA5-26114) in IHC
Immunohistochemistry with anti-POLR1D Polyclonal Antibody (PA5-26114)
Immunohistochemical analysis in formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated to the secondary antibody, followed by DAB staining.
POLR1D Antibody (PA5-26114) in WB
Western Blot with anti-POLR1D Polyclonal Antibody (PA5-26114)