PRL Antibody
PRL Polyclonal Antibody for IF, IHC, Western blot
| Details | |
| Host / Isotype: | Rabbit / IgG |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) |
| Immunogen: | Synthetic peptide from the S163 region of human PRL conjugated to KLH. |
| Ordering Information | ||||
| Pierce PRL Antibody |
| Storage: | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Form: | 100 ug of antibody purified through a protein A column followed by peptide affinity purification (0.25 mg/ml) in PBS with 0.09% (W/V) sodium azide. |
| Applications | Dilution * |
| Western Blot (WB) | 1:100-500 |
| Immunohistochemistry (IHC) | 1:50-100 |
| Immunofluorescence (IF) | 1:10-50 |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA5-26006 targets PRL in IF, IHC, and WB applications and shows reactivity with Human samples. The PA5-26006 immunogen is synthetic peptide from the S163 region of human PRL conjugated to KLH. |
| General Information |
| Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function. |
| Product Images |
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PRL Antibody (PA5-26006) in IF
Immunofluorescence with anti-PRL Polyclonal Antibody (PA5-26006)
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PRL Antibody (PA5-26006) in IHC
Immunohistochemistry with anti-PRL Polyclonal Antibody (PA5-26006)
Immunohistochemical analysis in formalin-fixed and paraffin-embedded human breast carcinoma followed by peroxidase-conjugated to the secondary antibody, followed by DAB staining. -
PRL Antibody (PA5-26006) in WB
Western Blot with anti-PRL Polyclonal Antibody (PA5-26006)
Western blot analysis of PRL in 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRL gene (Lane 2) .
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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PRL Antibodies > PRL Polyclonal Antibody PA5-26006
