Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Form:
100 ug of antibody purified through a protein A column followed by peptide affinity purification (0.25 mg/ml) in PBS with 0.09% (W/V) sodium azide.
Applications
Dilution *
Western Blot (WB)
1:100-500
Immunohistochemistry (IHC)
1:50-100
Flow Cytometry (FACS)
1:10-50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-25260 targets DLAT in FACS, IHC, and WB applications and shows reactivity with Human samples.
The PA5-25260 immunogen is synthetic peptide from the C-terminal region of human DLAT conjugated to KLH.
General Information
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1). dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrog
Product Images
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DLAT Antibody (PA5-25260) in FACS
Flow Cytometry with anti-DLAT Polyclonal Antibody (PA5-25260)
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.