Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Form:
100 ug of antibody purified through a protein A column followed by peptide affinity purification (0.25 mg/ml) in PBS with 0.09% (W/V) sodium azide.
Applications
Dilution *
Western Blot (WB)
1:100-500
Immunohistochemistry (IHC)
1:50-100
Flow Cytometry (FACS)
1:10-50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-24858 targets EFHC1 in FACS, IHC, and WB applications and shows reactivity with Human samples.
The PA5-24858 immunogen is synthetic peptide from the Central region of human EFHC1 conjugated to KLH.
General Information
Product Images
EFHC1 Antibody (PA5-24858) in FACS
Flow Cytometry with anti-EFHC1 Polyclonal Antibody (PA5-24858)
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
EFHC1 Antibody (PA5-24858) in IHC
Immunohistochemistry with anti-EFHC1 Polyclonal Antibody (PA5-24858)
Immunohistochemical analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
EFHC1 Antibody (PA5-24858) in WB
Western Blot with anti-EFHC1 Polyclonal Antibody (PA5-24858)