Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Form:
100 ug of antibody purified through a protein A column followed by peptide affinity purification (0.25 mg/ml) in PBS with 0.09% (W/V) sodium azide.
Applications
Dilution *
Western Blot (WB)
1:100-500
Immunohistochemistry (IHC)
1:50-100
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-24728 targets FA2H in IHC and WB applications and shows reactivity with Human and Murine samples.
The PA5-24728 immunogen is synthetic peptide from the Central region of human FA2H conjugated to KLH.
General Information
2-Hydroxysphingolipids are a subset of sphingolipids containing 2- hydroxy fatty acids. The 2-hydroxylation occurs during de novo ceramide synthesis and is catalyzed by fatty acid 2-hydroxylase (also known as fatty acid alpha-hydroxylase).
Product Images
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FA2H Antibody (PA5-24728) in WB
Western Blot with anti-FA2H Polyclonal Antibody (PA5-24728)
Immunohistochemical analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.