* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.1% BSA, 30% glycerol
0.05% sodium azide
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
PA5-23073 reacts with FUS in human samples. The target sequence has 95% homology to mouse and 91% homology to bovine.
PA5-23073 has been successfully used in Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Immunohistochemistry-Paraffin applications.
The PA5-23073 immunogen is a genomic peptide made to an N-terminal region of the human FUS protein (within residues 1-150).
The protein originally described as fused in Sarcoma is actually related to a number of different cellular processes and correlated to several diseases. FUS binds DNA as a single or double strand, and acts as a site for transcription factors to recognize. Since FUS is well-documented to be involved in liposarcoma, lipoma and leukaemia, it is believed that when its normal housekeeping functions related to transcription are disrupted, normal cells become cancerous. Interestingly, FUS is also associated with the fatal neuron disorder amyotrophic lateral sclerosis; interruption of its normal RNA-binding functions leads to incorrect cellular localization and leads to pathologies of the neuron. Thus, FUS regulation of cellular functions is a critical component of maintenance of healthy cells.
FUS Antibody (PA5-23073) in WB
Western Blot with anti-FUS Polyclonal Antibody (PA5-23073)