100 ul of Antigen affinity chromatography purified antibody (1 mg/ml) in PBS with 30% glycerol and 0.05% Sodium azide.
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-23052 reacts with Glucose Transporter GLUT4 in human and murine samples. This antibody is predicted to react with rat based on 100% sequence homology.
PA5-23052 has been successfully used in Western Blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry applications.
The PA5-23052 immunogen is a synthetic peptide made to an internal portion of the human Glucose Transporter GLUT4 protein (between residues 480-509).
Glucose Transporter 4 (GLUT4/SLC2A4) is a member of a family of proteins that facilitate glucose transport. In response to insulin GLUT4 translocates from low density microsomes to the plasma membrane. Activation of phosphatidylinositol-3-OH kinase is required for this trafficking event but is not sufficient. Insulin resistant subjects with and without diabetes show abnormal accumulation of GLUT4 in a dense membrane compartment in muscle and adipocytes from which insulin is unable to recruit GLUT4 to the cell surface.
Glucose Transporter GLUT4 Antibody (PA5-23052) in IF
Immunofluorescence with anti-Glucose Transporter GLUT4 Polyclonal Antibody (PA5-23052)