100 ul of Antigen affinity chromatography purified antibody (1.25 mg/ml) in PBS with 30% glycerol and 0.1% Sodium azide.
Immunohistochemistry (Paraffin) (IHC (P))
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-23036 reacts with Lactate Dehydrogenase A in human and murine samples. This antibody is predicted to react with bovine and porcine based on 100% sequence homology.
PA5-23036 has been successfully used in Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Immunohistochemistry-Paraffin applications.
The PA5-23036 immunogen is a synthetic peptide made to a C-terminal portion of the human Lactate Dehydrogenase A protein (within residues 280-332).
Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in a wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycolysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that causes deficiency in LDH-A has been implicated in exertional myoglobinuria (3).
Lactate Dehydrogenase A Antibody (PA5-23036) in WB
Western Blot with anti-Lactate Dehydrogenase A Polyclonal Antibody (PA5-23036)