HPRT antibody
HPRT Polyclonal antibody for ICC, IF, IHC (P), Western blot
Synonyms:
HGPRT, HPRT, HGPRTase, hypoxanthine-guanine phosphoribosyltransferase
Entrez Gene ID:
UniProt ID:
P00492
| Details | |
| Host / Isotype: | Rabbit / IgG |
| Class: | Polyclonal |
| Type: | antibody |
| Species Reactivity: | Human (Hu) |
| Immunogen: | Recombinant fragment corresponding to a region within amino acids 23 and 218 of HPRT (Uniprot ID#P00492) |
| Ordering Information | ||||
| Pierce HPRT antibody |
| Storage: | -20° C, Avoid Freeze/Thaw Cycles |
| Form: | 100 ul of antigen affinity purified antibody (0.95 mg/ml) in 0.1M Tris, 0.1M Glycine, pH 7, with 20% glycerol and 0.01% thimerosal. |
| Applications | Dilution * |
| Immunofluorescence (IF) | 1:100-1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100-1:500 |
| Western Blot (WB) | 1:500-1:3000 |
| Immunocytochemistry (ICC) | 1:100-1:500 |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA5-22281 targets HPRT in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples. The PA5-22281 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 23 and 195 (P00492) of HPRT. |
| General Information |
| Product Images |
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HPRT antibody (PA5-22281) in IF
Immunofluorescence with anti-HPRT Polyclonal antibody (PA5-22281)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using PA5-22281 anti-HPRT antibody at 1:200 dilution. -
HPRT antibody (PA5-22281) in IHC
Immunohistochemistry with anti-HPRT Polyclonal antibody (PA5-22281)
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft tissue using PA5-22281 anti-HPRT antibody at 1:100 dilution. -
HPRT antibody (PA5-22281) in WB
Western Blot with anti-HPRT Polyclonal antibody (PA5-22281)
Western blot analysis in whole cell lysates of (target) in lanes A). Molt-4 cells [30 ug] B). Raji cells [30 ug] using PA5-22281 anti-HPRT primary antibody at a dilution of 1:1000
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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HPRT Antibodies > HPRT Polyclonal antibody PA5-22281
