100 ul of antigen affinity purified antibody (1 mg/ml) in 0.1M Tris, 0.1M Glycine, pH 7, with 10% glycerol and 0.01% thimerosal.
Applications
Dilution *
Immunofluorescence (IF)
1:100-1:200
Immunohistochemistry (Paraffin) (IHC (P))
1:100-1:500
Western Blot (WB)
1:500-1:3000
Immunocytochemistry (ICC)
1:100-1:200
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-22276 targets FACA in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-22276 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 180 (O15360) of FANCA.
General Information
Product Images
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FACA antibody (PA5-22276) in IF
Immunofluorescence with anti-FACA Polyclonal antibody (PA5-22276)
Western blot analysis in whole cell lysates of (target) in lanes A). A431 cells [30 ug] B). Hela cells [30 ug] using PA5-22276 anti-FACA primary antibody at a dilution of 1:100