100 ul of antigen affinity purified antibody (0.92 mg/ml) in 0.1M Tris, 0.1M Glycine, pH 7, with 10% glycerol and 0.01% thimerosal.
Applications
Dilution *
Immunofluorescence (IF)
1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P))
1:100-1:1000
Western Blot (WB)
1:500-1:3000
Immunocytochemistry (ICC)
1:100-1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-22258 targets alpha Tubulin 4a in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and Murine samples.
The PA5-22258 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 21 and 203 (P68366) of alpha Tubulin 4a.
General Information
Product Images
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alpha Tubulin 4a antibody (PA5-22258) in WB
Western Blot with anti-alpha Tubulin 4a Polyclonal antibody (PA5-22258)
Western blot analysis in whole cell lysates of (target) in lanes A). A431 cells [30 ug] B). Molt-4 cells [30 ug] using PA5-22258 anti-alpha Tubulin 4a primary antibody at a dilution of 1:1000