100 ul of antigen affinity purified antibody (1 mg/ml) in 0.1M Tris, 0.1M Glycine, pH 7, with 10% glycerol and 0.01% thimerosal.
Applications
Dilution *
Immunofluorescence (IF)
1:100-1:200
Immunohistochemistry (Paraffin) (IHC (P))
1:100-1:500
Western Blot (WB)
1:1000-1:10000
Immunocytochemistry (ICC)
1:100-1:200
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-22178 targets Pyruvate Kinase(liver/RBC) in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-22178 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 220 (P30613) of Pyruvate Kinase (liver/RBC).
General Information
Product Images
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Pyruvate Kinase(liver/RBC) antibody (PA5-22178) in IF
Immunofluorescence with anti-Pyruvate Kinase(liver/RBC) Polyclonal antibody (PA5-22178)
Western blot analysis in whole cell lysates of (target) in lanes A). H1299 cells [30 ug] B). Hela cells [30 ug] using PA5-22178 anti-Pyruvate Kinase(liver/RBC) primary antibody at a dilution of 1:1000