100 ul of antigen affinity purified antibody (0.57 mg/ml) in 0.1M Tris, 0.1M Glycine, pH 7, with 10% glycerol and 0.01% thimerosal.
Applications
Dilution *
Immunofluorescence (IF)
1:100-1:500
Immunohistochemistry (Paraffin) (IHC (P))
1:100-1:500
Western Blot (WB)
1:500-1:3000
Immunocytochemistry (ICC)
1:100-1:500
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-21390 targets ribosomal protein S10 in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and Murine samples.
The PA5-21390 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 151 (P46783) of RPS10.
General Information
Product Images
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ribosomal protein S10 antibody (PA5-21390) in WB
Western Blot with anti-ribosomal protein S10 Polyclonal antibody (PA5-21390)
Western blot analysis in A431 whole cell lysate [30 ug] of ribosomal protein S10 using PA5-21390 anti-ribosomal protein S10 antibody at a dilution of 1:1000
Western blot analysis in Mouse brain cell lysate [50 ug] of ribosomal protein S10 using PA5-21390 anti-ribosomal protein S10 antibody at a dilution of 1:1000