100 ul of antigen affinity purified antibody (1 mg/ml) in 0.1M Tris, 0.1M Glycine, pH 7, with 10% glycerol and 0.01% thimerosal.
Applications
Dilution *
Immunohistochemistry (Paraffin) (IHC (P))
1:100-1:500
Western Blot (WB)
1:500-1:3000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-21326 targets HPA2 in IHC (P) and WB applications and shows reactivity with Human and Murine samples.
The PA5-21326 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 260 and 542 (Q8WWQ2) of HPA2.
General Information
Product Images
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HPA2 antibody (PA5-21326) in IHC
Immunohistochemistry with anti-HPA2 Polyclonal antibody (PA5-21326)
Western blot analysis in whole cell lysates of (target) in lanes A). 293T cells [30 ug] B). H1299 cells [30 ug] C). HeLa S3 cells [30 ug] using PA5-21326 anti-HPA2 primary antibody at a dilution of 1:1000