NCF4/P40PHOX Antibody
NCF4/P40PHOX Polyclonal Antibody for IHC, Western blot
Synonyms:
NCF4; P40PHOX; neutrophil cytosolic factor 4, 40kDa ; CTA-833B7.1; MGC3810; NCF; SH3PXD4; OTTHUMP00000028736; OTTHUMP00000043756; neutrophil NADPH oxidase factor 4; neutrophil cytosol factor 4; neutrophil cytosolic factor 4 (40kD)
Entrez Gene ID:
UniProt ID:
(Hu) Q15080
| Details | |
| Host / Isotype: | Goat |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) |
| Immunogen: | Synthetic peptide sequence (KDFPEEDDPTN) corresponding to the internal amino acids of NCF4 |
| Ordering Information | ||||
| Pierce NCF4/P40PHOX Antibody |
| Storage: | -20° C, Avoid Freeze/Thaw Cycles |
| Form: | 100 ug of purified antibody from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Buffered in 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Applications | Dilution * |
| Immunohistochemistry (IHC) | 2-4µg/ml |
| Western Blot (WB) | 0.3-1 ug/ml |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA5-18751 targets NCF4/P40PHOX in IHC and WB applications and shows reactivity with Human samples. The PA5-18751 immunogen is synthetic peptide sequence (KDFPEEDDPTN) corresponding to the internal amino acids of NCF4. |
| General Information |
| The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 to form a complex with neutrophil cytosolic factor 1 kinase, which suggests its role in PIkinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
| Product Images |
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NCF4/P40PHOX Antibody (PA5-18751) in WB
Western Blot with anti-NCF4/P40PHOX Polyclonal Antibody (PA5-18751)
Western Blot staining of Daudi cell lysate using PA5-18751 at a concentration of 0.5 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence. -
NCF4/P40PHOX Antibody (PA5-18751) in WB
Western Blot with anti-NCF4/P40PHOX Polyclonal Antibody (PA5-18751)
Western blot of HEK293 overexpressing P40PHOX using PA5-18751, mock transfection as a control in first lane. -
NCF4/P40PHOX Antibody (PA5-18751) in IHC
Immunohistochemistry with anti-NCF4/P40PHOX Polyclonal Antibody (PA5-18751)
Immunohistochemical staining of paraffin embedded of Human Tonsil using PA5-18751 at a concentration of 2.5 ug/ml. The tissue was processed by steamed antigen retrieval with citrate buffer pH 6 and stained with AP.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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NCF4/P40PHOX Antibodies > NCF4/P40PHOX Polyclonal Antibody PA5-18751
