Pyruvate Dehydrogenase Antibody
Pyruvate Dehydrogenase Polyclonal Antibody for ICC, Western blot
Synonyms:
PDHE1-A type I; pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; pyruvate dehydrogenase complex, E1-alpha polypeptide 1
Entrez Gene ID:
UniProt ID:
(Hu) P08559, (Ms) P35486
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Mouse (Ms) Rat (Rt) Non-human primate (Nhp) |
| Immunogen: | Synthetic peptide corresponding to the sequences of both alpha1 and alpha2 subunits of human pyruvate dehydrogenase. |
| Ordering Information | ||||
| Pierce Pyruvate Dehydrogenase Antibody |
| Storage: | -20°C |
| Form: | 100 ul of antibody in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol. |
| Applications | Dilution * |
| Immunocytochemistry (ICC) | 1:25 |
| Western Blot (WB) | 1:1000 |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA5-17190 targets Pyruvate Dehydrogenase in ICC, and WB applications and shows reactivity with Human, Murine, Non-human primate, and Rat samples. The PA5-17190 immunogen is synthetic peptide corresponding to the sequences of both alpha1 and alpha2 subunits of human pyruvate dehydrogenase. PA5-17190 detects Pyruvate Dehydrogenase which has a predicted molecular weight of approximately 43 kDa. |
| General Information |
| The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
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Pyruvate Dehydrogenase Antibody (PA5-17190) in IF
Immunofluorescence with anti-Pyruvate Dehydrogenase Polyclonal Antibody (PA5-17190)
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Pyruvate Dehydrogenase Antibody (PA5-17190) in IF
Immunofluorescence with anti-Pyruvate Dehydrogenase Polyclonal Antibody (PA5-17190)
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Pyruvate Dehydrogenase Antibody (PA5-17190) in IF
Immunofluorescence with anti-Pyruvate Dehydrogenase Polyclonal Antibody (PA5-17190)
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Pyruvate Dehydrogenase Antibody (PA5-17190) in WB
Western Blot with anti-Pyruvate Dehydrogenase Polyclonal Antibody (PA5-17190)
Western blot analysis of Pyruvate Dehydrogenase in extracts from HepG2 and 293 cells using Pyruvate Dehydrogenase polyclonal antibody (Product # PA5-17190).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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Pyruvate Dehydrogenase Antibodies > Pyruvate Dehydrogenase Polyclonal Antibody PA5-17190
