100 ul of antibody in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol.
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-17025 targets Myosin IIa in ICC, and WB applications and shows reactivity with Human, Murine, and Rat samples.
The PA5-17025 immunogen is synthetic peptide corresponding to the carboxy terminus of mouse myosin IIa.
PA5-17025 detects Myosin IIa which has a predicted molecular weight of approximately 230 kDa.
This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness.
Myosin IIa Antibody (PA5-17025) in WB
Western Blot with anti-Myosin IIa Polyclonal Antibody (PA5-17025)
Immunofluorescent analysis of Myosin IIa in HeLa cells using a Myosin IIa polyclonal antibody (Product # PA5-17025) (green) showing colocalization with actin filaments that have been labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.