Myosin IIa Antibody
Myosin IIa Polyclonal Antibody for ICC, Western blot
Synonyms:
MYH9 variant protein; NMMHC II-a; NMMHC-A; NMMHC-IIA; cellular myosin heavy chain, type A; myosin heavy chain 9; myosin heavy chain, non-muscle IIa; myosin-9; non-muscle myosin heavy chain A; non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A
Entrez Gene ID:
UniProt ID:
(Hu) P35579, (Ms) Q8VDD5, (Rt) Q62812
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Mouse (Ms) Rat (Rt) |
| Immunogen: | Synthetic peptide corresponding to the carboxy terminus of mouse myosin IIa. |
| Ordering Information | ||||
| Pierce Myosin IIa Antibody |
| Storage: | -20°C |
| Form: | 100 ul of antibody in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol. |
| Applications | Dilution * |
| Immunocytochemistry (ICC) | 1:50 |
| Western Blot (WB) | 1:1000 |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA5-17025 targets Myosin IIa in ICC, and WB applications and shows reactivity with Human, Murine, and Rat samples. The PA5-17025 immunogen is synthetic peptide corresponding to the carboxy terminus of mouse myosin IIa. PA5-17025 detects Myosin IIa which has a predicted molecular weight of approximately 230 kDa. |
| General Information |
| This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. |
| Product Images |
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Myosin IIa Antibody (PA5-17025) in WB
Western Blot with anti-Myosin IIa Polyclonal Antibody (PA5-17025)
Western blot analysis of Myosin IIa in extracts from Hela and HT-29 cells using Myosin IIa polyclonal antibody (Product # PA5-17025). -
Myosin IIa Antibody (PA5-17025) in WB
Western Blot with anti-Myosin IIa Polyclonal Antibody (PA5-17025)
Western blot analysis of Myosin IIa in extracts from COS cells, mock transfected or transfected with nonmuscle EGFP-myosin IIa or IIb using Myosin IIa polyclonal antibody (Product # PA5-17025). -
Myosin IIa Antibody (PA5-17025) in IF
Immunofluorescence with anti-Myosin IIa Polyclonal Antibody (PA5-17025)
Immunofluorescent analysis of Myosin IIa in HeLa cells using a Myosin IIa polyclonal antibody (Product # PA5-17025) (green) showing colocalization with actin filaments that have been labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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Myosin IIa Antibodies > Myosin IIa Polyclonal Antibody PA5-17025
