500 ul of total IgG purified from rabbit anti-serum by Protein A chromatography (4 mg/ml). Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Immunohistochemistry (Paraffin) (IHC (P))
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-16458 targets CD117 in IHC (P) applications and shows reactivity with Human samples.
The PA5-16458 immunogen is a synthetic peptide from C-terminus of human CD117/c-kit protein.
c-kit is a transmembrane receptor tyrosine kinase. It binds to stem cell factor (SCF). It is expressed in many tissues and cells. C-kit/SCf is involved in development of several lineages of stem cells, such as germ cells, neural crest-derived melanocytes and hematopoietic precursor cells Antibody to CD117/c-kit may be helpful in the histopathological identification of gastrointestinal stromal tumors (GIST).
CD117 Antibody (PA5-16458) in IHC
Immunohistochemistry with anti-CD117 Polyclonal Antibody (PA5-16458)