* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
PA5-14713 targets PDGFRA in Western blot and immunohistochemistry applications and shows reactivity with Human samples.
The PA5-14713 immunogen is a synthetic peptide surrounding Y754 of PDGFRA conjugated to KLH.
PA5-14713 detects PDGFRA which has a predicted molecular weight of approximately 123 kDa.
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
PDGF Receptor alpha/CD140a Antibody (PA5-14713) in IHC
Immunohistochemistry with anti-PDGF Receptor alpha/CD140a Polyclonal Antibody (PA5-14713)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human hepatocarcinoma tissue using anti-PDGFRA polyclonal antibody (PA5-14713) and then the tissue was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
PDGF Receptor alpha/CD140a Antibody (PA5-14713) in WB
Western Blot with anti-PDGF Receptor alpha/CD140a Polyclonal Antibody (PA5-14713)
Western blot analysis of PDGFRA using anti-PDGFRA polyclonal antibody (PA5-14713) in 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDGFRA gene (Lane 2).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)