Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
100 µg (0.25 mg/ml) in PBS with 0.09% (W/V) sodium azide.
Flow Cytometry (FACS)
1:10 - 1:50
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-14667 targets FLT3 in flow cytometry, Western blot, and immunohistochemistry applications and shows reactivity with Human samples.
The PA5-14667 immunogen is a synthetic peptide from the N-terminal region of FLT3 conjugated to KLH.
PA5-14667 detects FLT3 which has a predicted molecular weight of approximately 113 kDa.
This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
FLT3 Antibody (PA5-14667) in WB
Western Blot with anti-FLT3 Polyclonal Antibody (PA5-14667)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human cancer tissue using anti-FLT3 polyclonal antibody (PA5-14667) and then the tissue was peroxidase-conjugated to the secondary antibody, followed by DAB staining.