Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
100 µg (0.25 mg/ml) in PBS with 0.09% (W/V) sodium azide.
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-11896 targets PDX1 in WB and IHC applications and shows reactivity with Human samples. The PA5-11896 immunogen is a synthetic peptide from from the C-terminal region of PDX1 conjugated to KLH. PA5-11896 detects PDX1 which has a predicted molecular weight of approximately 54 kDa.
The pyruvate dehydrogenase complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein. This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Defects in this gene are a cause of pyruvate dehydrogenase deficiency which results in neurological dysfunction and lactic acidosis in infancy and early childhood. This protein is also a minor antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis. In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC eventually leads to cirrhosis and liver failure. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
PDX1 Antibody (PA5-11896) in WB
Western Blot with anti-PDX1 Polyclonal Antibody (PA5-11896)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human cancer tissue using anti-PDX1 polyclonal antibody (PA5-11896) and then the tissue was peroxidase-conjugated to the secondary antibody, followed by DAB staining.