PDX-1 Antibody
PDX-1 Polyclonal Antibody for IF, IHC, Western blot
Synonyms:
Pyruvate Dehydrogenase Complex Component-X, PDHX, E3BP, DLDBP, Pyruvate Dehydrogenase Complex Lipoyl-Containing Component X
Entrez Gene ID:
UniProt ID:
(Hu) P52945, (Ms) P52946
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Mouse (Ms) |
| Immunogen: | Synthetic Peptide: Q(21) R G P A P E F S A S P P A C(35) |
| Ordering Information | ||||
| Pierce PDX-1 Antibody |
| Storage: | -20° C, Avoid Freeze/Thaw Cycles |
| Form: | 100 ul of antiserum containing 0.05% sodium azide. |
| Applications | Dilution * |
| Immunofluorescence (IF) | 1:500 |
| Immunohistochemistry (IHC) | 1:500 |
| Western Blot (WB) | 1:3,000 |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA3-830 detects human and mouse pdx-1 alpha and shows no cross-reactivity to other pdx isoforms. PA3-830 has been successfully used in Western blot, immunohistochemical, and immunofluorescence procedures. By Western blot, this antibody detects an ~35 kDa protein. PA3-830 immunogen is a KLH conjugated synthetic peptide corresponding to amino acid residues 21-35 from human pdx-1 protein. This antibody is generated against the N-terminal portion of the protein, while PA3-831 is C-terminal. The PA3-830 antibody recognizes both cytoplasmic and nuclear proteins. Formalin-fixed tissues can cause high background, so counterstaining with another antibody which is mainly expressed in the cytoplasm (i.e. insulin) is recommended. |
| General Information |
| pdx-1 (pancreas duodenum homeobox-1, also known as IPF-1, IDX-1, and STF-1) is a transcription factor that has been shown to activate insulin, glucokinase, IAPP, and somatostatin gene transcription. Significant interest surrounds this proteins glucose-dependent regulation of insulin gene transcription. Additionally, pdx-1 has been studied for its role in pancreatic development which includes maturation and differentiation of pancreatic precursor cells. Mutations in the pdx-1 gene have been shown to cause pancreatic agenesis, maturity-onset diabetes of the young, and type II diabetes. |
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PDX-1 Antibody (PA3-830) in IHC
Immunohistochemistry with anti-PDX-1 Polyclonal Antibody (PA3-830)
Immunohistochemistry was performed on normal biopsies of deparaffinized human pancreas tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a Rabbit Polyclonal Antibody recognizing PDX-1 (PA3-830 ) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
PDX-1 Antibody (PA3-830) in IHC
Immunohistochemistry with anti-PDX-1 Polyclonal Antibody (PA3-830)
Immunohistochemistry was performed on normal biopsies of deparaffinized human stomach tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:500 with a Rabbit Polyclonal Antibody recognizing PDX-1 (PA3-830 ) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
PDX-1 Antibody (PA3-830) in IHC
Immunohistochemistry with anti-PDX-1 Polyclonal Antibody (PA3-830)
Immunohistochemistry was performed on normal biopsies of deparaffinized human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a Rabbit Polyclonal Antibody recognizing PDX-1 (PA3-830 ) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
PDX-1 Antibody (PA3-830) in IF
Immunofluorescence with anti-PDX-1 Polyclonal Antibody (PA3-830)
Immunofluorescent analysis of PDX-1 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a PDX-1 polyclonal antibody (Product # PA3-830) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). PDX-1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification. -
PDX-1 Antibody (PA3-830) in IF
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Immunofluorescence with anti-PDX-1 Polyclonal Antibody (PA3-830)
Immunofluorescent staining of pdx-1 in mouse islet cells using PA3-830 and counterstaining (merge) with insulin. The PA3-830 antibody recognizes both cytoplasmic and nuclear proteins. Formalin-fixed tissues can cause high background, so counterstaining with another antibody which is mainly expressed in the cytoplasm (i.e. insulin) is recommended. -
PDX-1 Antibody (PA3-830) in WB
Western Blot with anti-PDX-1 Polyclonal Antibody (PA3-830)
Western blot of pdx-1 in human pancreatic cancer cells MIA-1, PANC-1 and CAPAN-1 using PA3-830.
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| PubMed References: |
| Immunohistochemistry | ||
| Species / Dilution | Summary | |
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Hu / 1:100 |
PA3-830 was used in immunohistochemistry to investigate the expression of PDX-1 in human cancers through tissue microarray analyses
World J Surg. 2005 Mar;29(3):334-8.
"Tissue MicroArray analyses of pancreatic duodenal homeobox-1 in human cancers." Author(s): Wang XP, Li ZJ, Magnusson J, Brunicardi FC Number of Citations: 1 (See PubMed article  )
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(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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PDX-1 Antibodies > PDX-1 Polyclonal Antibody PA3-830
