GAPDH Antibody
GAPDH Polyclonal Antibody for Western blot
Synonyms:
Glyceraldehyde-3-Phosphate Dehydrogenase, GAPD, G3PD, G3PDH
Entrez Gene ID:
UniProt ID:
(Hu) P04406, (Ms) P16858, (Rt) P04797
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Mouse (Ms) Rat (Rt) |
| Immunogen: | Synthetic peptide: V(4) K V G V N G F G R I G R L V(18) |
| Ordering Information | ||||
| Pierce GAPDH Antibody |
| Storage: | -20° C, Avoid Freeze/Thaw Cycles |
| Form: | 100 ug of epitope affinity purified rabbit IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution * |
| Western Blot (WB) | 1 µg/ml |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA1-988 detects rat GAPDH in human HeLa cell lysates and mouse and rat tissues.. PA1-988 has been used successfully in Western blotting. By Western blot this antibody specifically detects a ~36 kDa protein representing GAPDH. PA1-988 immunizing peptide corresponds to amino acid residues 4-18 from human GAPDH. |
| General Information |
| Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in western blot analysis. Additionally, the antigen is conserved in most eukaryotes. |
| Product Images |
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GAPDH Antibody (PA1-988) in WB
Western Blot with anti-GAPDH Polyclonal Antibody (PA1-988)
Western blot of rat heart protein extract using PA1-988.
| PubMed References: |
| Western Blot | ||
| Species / Dilution | Summary | |
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Ms / 1:2,000 |
PA1-988 was used in western blot to study the effect of rimonabant on enzyme content of mouse 3T3 F442A preadipocytes
Mol Pharmacol. 2006 Feb;69(2):471-8.
"The cannabinoid CB1 receptor antagonist rimonabant (SR141716) inhibits cell proliferation and increases markers of adipocyte maturation in cultured mouse 3T3 F442A preadipocytes." Author(s): Gary-Bobo M, Elachouri G, Scatton B, Le Fur G, Oury-Donat F, Bensaid M Number of Citations: 1 (See PubMed article  )
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Ms / Not Cited |
PA1-988 was used in western blot to perform the global proteomics surveys of cardiac ventricle of a phospholamban mutant mouse
Mol Cell Proteomics. 2008 Mar;7(3):519-33.
"Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses." Author(s): Gramolini AO, Kislinger T, Alikhani-Koopaei R, Fong V, Thompson NJ, Isserlin R, Sharma P, Oudit GY, Trivieri MG, Fagan A, Kannan A, Higgins DG, Huedig H, Hess G, Arab S, Seidman JG, Seidman CE, Frey B, Perry M, Backx PH, Liu PP, MacLennan DH, Emili A Number of Citations: 1 (See PubMed article )
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(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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GAPDH Antibodies > GAPDH Polyclonal Antibody PA1-988
