Parvalbumin Antibody
Parvalbumin Polyclonal Antibody for ELISA, IHC (P), IP, Western blot
| Details | |
| Host / Isotype: | Rabbit / IgG |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Rat (Rt) |
| Immunogen: | Purified parvalbumin from rat skeletal muscle. |
| Ordering Information | ||||
| Pierce Parvalbumin Antibody |
| Storage: | -20° C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µg of immunogen affinity purified IgG, lyophilized from PBS containing 15 mg/ml BSA and 0.05% sodium azide. Resuspend in 100 µl dH2O for 1 mg/ml concentration. |
| Applications | Dilution * |
| Immunoprecipitation (IP) | 5 µg/ml |
| ELISA (ELISA) | Assay dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/ml |
| Immunofluorescence (IF) | 1:100-1:200 |
| Western Blot (WB) | 0.1 µg/ml |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA1-933 detects parvalbumin protein in human and rat samples. PA1-933 has successfully been used in Western blot, ELISA, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 12 kDa protein representing parvalbumin from rat cerebellum. Parvalbumin protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane. The PA1-933 immunizing protein corresponds to purified parvalbumin from rat skeletal muscle. |
| General Information |
| Parvalbumin (PV) is a calcium binding protein expressed in specific muscle fibers and fast-firing neurons. PV consists of a single, unbranched chain of linked amino acids and belongs to a larger group of EF hand proteins. Studies have demonstrated that parvalbumin acts in the decay of calcium in the contraction/ relaxation cycle of fast twitch muscles. This data has shown a positive correlation between the rate of relaxation and the concentration of parvalbumin. Parvalbumin is also expressed in a specific population of GABAergic interneurones which are thought to play a role in maintaining the balance between excitation and inhibition in the cortex as well as the hippocampus. In amyotrophic lateral sclerosis (ALS) patents, parvalbumin immunoreactivity is specifically absent from neuron populations lost early in ALS. |
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Parvalbumin Antibody (PA1-933) in IF
Immunofluorescence with anti-Parvalbumin Polyclonal Antibody (PA1-933)
Immunofluorescent analysis of Parvalbumin using Anti-Parvalbumin Polyclonal Antibody (Product# PA1-933) shows staining in C6 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin (Product# PA1-933) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552, Goat Anti-Rabbit). Images were taken at 60X magnification. -
Parvalbumin Antibody (PA1-933) in IF
Immunofluorescence with anti-Parvalbumin Polyclonal Antibody (PA1-933)
Immunofluorescent analysis of Parvalbumin using Anti-Parvalbumin Polyclonal Antibody (Product# PA1-933) shows staining in Hela Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin (Product# PA1-933) at a dilution of 1:100 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552, Goat Anti-Rabbit). Images were taken at 60X magnification. -
Parvalbumin Antibody (PA1-933) in IF
Immunofluorescence with anti-Parvalbumin Polyclonal Antibody (PA1-933)
Immunofluorescent analysis of Parvalbumin using Anti-Parvalbumin Polyclonal Antibody (Product# PA1-933) shows staining in U251 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin (Product# PA1-933) at a dilution of 1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552, Goat Anti-Rabbit). Images were taken at 60X magnification. -
Parvalbumin Antibody (PA1-933) in IHC
Immunohistochemistry with anti-Parvalbumin Polyclonal Antibody (PA1-933)
Immunohistochemistry was performed on normal biopsies of deparaffinized Human cerebellum tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin (PA1-933) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
Parvalbumin Antibody (PA1-933) in IHC
Immunohistochemistry with anti-Parvalbumin Polyclonal Antibody (PA1-933)
Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing Parvalbumin (PA1-933) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
Parvalbumin Antibody (PA1-933) in IHC
Immunohistochemistry with anti-Parvalbumin Polyclonal Antibody (PA1-933)
Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin (PA1-933) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
Parvalbumin Antibody (PA1-933) in WB
Western Blot with anti-Parvalbumin Polyclonal Antibody (PA1-933)
Western blot of parvalbumin from rat cerebellum extract using PA1-933.
| PubMed References: |
| ELISA | ||
| Species / Dilution | Summary | |
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Hu / Not Cited |
PA1-933 was used in ELISA and western blot to compare the expression levels of parvalbumin in parahippocampal gyrus in Alzheimer disease patients and normal people
J Neurol Sci. 1992 Jul;110(1-2):57-61.
"Immunoreactive parvalbumin concentrations in parahippocampal gyrus decrease in patients with Alzheimer's disease." Author(s): Inaguma Y, Shinohara H, Inagaki T, Kato K Number of Citations: 2 (See PubMed article  )
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Rt / Not Cited |
PA1-933 was used in ELISA and western blot to investigate tissue distribution of parvalbumin in rat and its changes during development
Biochim Biophys Acta. 1991 Sep 2;1075(1):68-74.
"Sensitive immunoassay for rat parvalbumin: tissue distribution and developmental changes." Author(s): Inaguma Y, Kurobe N, Shinohara H, Kato K Number of Citations: 3 (See PubMed article )
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| Immunohistochemistry | ||
| Species / Dilution | Summary | |
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Ms / Not Cited |
PA1-933 was used in immunohistochemistry to study the neuronal plasticity in murine barrel cortex during development
Eur J Neurosci. 2009 Dec 3;30(11):2053-63.
"Parvalbumin-containing neurons, perineuronal nets and experience-dependent plasticity in murine barrel cortex." Author(s): Nowicka D, Soulsby S, Skangiel-Kramska J, Glazewski S Number of Citations: 5 (See PubMed article )
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Rt / Not Cited |
PA1-933 was used in immunohistochemistry to detect the expression of Nogo-A protein after ischemic stroke in rat
Stroke. 2008 Jul;39(7):2091-8.
"Nogo-A expression after focal ischemic stroke in the adult rat." Author(s): Cheatwood JL, Emerick AJ, Schwab ME, Kartje GL Number of Citations: 1 (See PubMed article )
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Rt / 1:1000 |
PA1-933 was used in immunohistochemistry to investigate the changes of endonuclease G in thalamic reticular nucleus neurons after ischemia
Exp Brain Res. 2008 Sep;190(1):81-9.
"Endonuclease G expression in thalamic reticular nucleus after global cerebral ischemia." Author(s): Nielsen M, Zimmer J, Diemer NH Number of Citations: 1 (See PubMed article )
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Rt / 1:100 |
PA1-933 was used in immunohistochemistry to investigate the effect of growth factors on neuron proliferation and growth after ischemia
J Neurosurg. 2010 Oct;113(4):835-50.
"Induction of striatal neurogenesis and generation of region-specific functional mature neurons after ischemia by growth factors. Laboratory investigation." Author(s): Yoshikawa G, Momiyama T, Oya S, Takai K, Tanaka J, Higashiyama S, Saito N, Kirino T, Kawahara N Number of Citations: 0 (See PubMed article )
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Rt / Not Cited |
PA1-933 was used in immunohistochemistry to study the ataxic Guillain-Barr syndrome and acute sensory ataxic neuropathy
J Neurol Neurosurg Psychiatry. 2011 Mar;82(3):294-9.
"Ataxic Guillain-Barré syndrome and acute sensory ataxic neuropathy form a continuous spectrum." Author(s): Ito M, Matsuno K, Sakumoto Y, Hirata K, Yuki N Number of Citations: 1 (See PubMed article )
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Su / 0.2 ug/ml |
PA1-933 was used in immunohistochemistry to investigate the central nervous system of sea cucumber
Glia. 2010 Oct;58(13):1581-93.
"Organization of glial cells in the adult sea cucumber central nervous system." Author(s): Mashanov VS, Zueva OR, Garcia-Arraras JE Number of Citations: 1 (See PubMed article )
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| Western Blot | ||
| Species / Dilution | Summary | |
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Hu / 0.05 ug/ml |
PA1-933 was used in ELISA and western blot to compare the expression levels of parvalbumin in parahippocampal gyrus in Alzheimer disease patients and normal people
J Neurol Sci. 1992 Jul;110(1-2):57-61.
"Immunoreactive parvalbumin concentrations in parahippocampal gyrus decrease in patients with Alzheimer's disease." Author(s): Inaguma Y, Shinohara H, Inagaki T, Kato K Number of Citations: 2 (See PubMed article )
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Rt / 0.06 ug/ml |
PA1-933 was used in ELISA and western blot to investigate tissue distribution of parvalbumin in rat and its changes during development
Biochim Biophys Acta. 1991 Sep 2;1075(1):68-74.
"Sensitive immunoassay for rat parvalbumin: tissue distribution and developmental changes." Author(s): Inaguma Y, Kurobe N, Shinohara H, Kato K Number of Citations: 3 (See PubMed article )
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Rt / 1:200 |
PA1-933 was used in western blot to study the effects of ageing on muscle quality and sarcoplasmic reticulum Ca(2+) release.
Acta Physiol (Oxf). 2011 Mar;201(3):391-403.
"Ageing, but not yet senescent, rats exhibit reduced muscle quality and sarcoplasmic reticulum function." Author(s): Russ DW, Grandy JS, Toma K, Ward CW Number of Citations: 3 (See PubMed article )
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(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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Parvalbumin Antibodies > Parvalbumin Polyclonal Antibody PA1-933
