100 µg of epitope affinity-purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide.
Western Blot (WB)
0.5 - 1.0 µg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA1-880 detects DNA methyltransferase 1 (Dmnt1) from HeLa cell nuclear extracts.
PA1-880 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~180 kDa protein representing Dnmt1 from HeLa cell nuclear extract. A doublet between 40 and 45 kDa is also seen on Western blots using this antibody.
The PA1-880 immunogen is a synthetic peptide corresponding to residues A(171) K G P A K R K P Q E E S E(185) of human Dnmt1. PA1-880 immunizing peptide (Cat. # PEP-136) is available for use in neutralization and control experiments.
DNA methyltransferases (Dnmts) methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, genetic imprinting, and X-chromosome inactivation. Hypermethylation of DNA has been implicated in tumorigenesis through improper methylation of growth regulatory gene promoter regions. There exist three isoforms of Dnmts, Dnmt1, 2 and 3. Dnmt1 is believed to perform most of the maintenance and de novo methylation activities that occur in somatic cells of mammals. Studies have shown that Dnmt1 can establish repression of transcription complexes consisting of Dnmt1, histone deacetylase 2 (HDAC2), and DNA methyltransferase 1 associated protein (DMAP1). Therefore, in addition to DNA methylation maintenance, Dnmt1 is thought to directly target transcriptionally repressive chromatin to the genome during S-phase DNA replication.
DNA Methyltransferase 1 Antibody (PA1-880) in WB
Western Blot with anti-DNA Methyltransferase 1 Polyclonal Antibody (PA1-880)
PA1-880 was used in western blot to investigate a DNA methyltransferase 1 isoform's expression level during the differentiation of muscle
Cell Growth Differ. 2000 Oct;11(10):551-9. "Expression of an alternative Dnmt1 isoform during muscle differentiation."
Author(s): Aguirre-Arteta AM, Grunewald I, Cardoso MC, Leonhardt H
Number of Citations: 4
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)