LAMP2 Antibody
LAMP2 Polyclonal Antibody for IF, IHC, Western blot
Synonyms:
Lysosome-Associated Membrane Protein-2, LAMPB
Entrez Gene ID:
UniProt ID:
(Hu) P13473, (Ms) P17047
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Mouse (Ms) Rat (Rt) |
| Immunogen: | Synthetic Peptide: C G(400) L K R H H T G Y E Q F(411) |
| Ordering Information | ||||
| Pierce LAMP2 Antibody |
| Storage: | -20° C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µl of epitope affinity purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution * |
| Immunofluorescence (IF) | Assay dependent |
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:20 |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA1-655 detects lysosome-associated membrane protein-2 (LAMP2) from rat, mouse and human tissues. PA1-655 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~110 kDa protein representing LAMP2 from purified lysosomes from L cells. PA1-655 immunizing peptide corresponds to amino acid residues 400-411 from rat LAMP2. This sequence is completely conserved between rat, mouse, and guinea pig LAMP2. PA1-655 immunizing peptide (Cat. # PEP-039) is available for use in neutralization and control experiments. |
| General Information |
| Lysosomes are membrane-bound vacuoles which play a critical role in cellular metabolism. These organelles participate in endocytosis, the synthesis and assembly of acid hydrolases, act as sites for digestion of foreign materials and for specialized autolytic cellular processes. Membrane-associated glycoproteins have an important role in normal lysosomal function. Prior to posttranslational modification, Lysosome-associated membrane protein-2 (LAMP-2) is an ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of ~100-110 kDa. LAMP2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. |
| Product Images |
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LAMP2 Antibody (PA1-655) in IHC
Immunohistochemistry with anti-LAMP2 Polyclonal Antibody (PA1-655)
Immunohistochemistry was performed on normal deparaffinized Human placenta tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing LAMP2 (PA1-655) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
LAMP2 Antibody (PA1-655) in IHC
Immunohistochemistry with anti-LAMP2 Polyclonal Antibody (PA1-655)
Immunohistochemistry was performed on cancer biopsies of deparaffinized Human prostate carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing LAMP2 (PA1-655) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
LAMP2 Antibody (PA1-655) in IHC
Immunohistochemistry with anti-LAMP2 Polyclonal Antibody (PA1-655)
Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing LAMP2 (PA1-655) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
LAMP2 Antibody (PA1-655) in IF
Immunofluorescence with anti-LAMP2 Polyclonal Antibody (PA1-655)
Immunofluorescent analysis of LAMP2 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a LAMP2 polyclonal antibody (Product # PA1-655) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). LAMP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification. -
LAMP2 Antibody (PA1-655) in IF
Immunofluorescence with anti-LAMP2 Polyclonal Antibody (PA1-655)
Immunofluorescent analysis of LAMP2 in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a LAMP2 polyclonal antibody (Product # PA1-655) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). LAMP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
| PubMed References: |
| Immunocytochemistry | ||
| Species / Dilution | Summary | |
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Hu / Not Cited |
PA1-655 was used in immunocytochemistry to investigate the relationship between macroautophagy and apoptosis in mammalian cells.
Mol Cell Biol. 2005 Feb;25(3):1025-40.
"Inhibition of macroautophagy triggers apoptosis." Author(s): Boya P, González-Polo RA, Casares N, Perfettini JL, Dessen P, Larochette N, Métivier D, Meley D, Souquere S, Yoshimori T, Pierron G, Codogno P, Kroemer G Number of Citations: 1 (See PubMed article  )
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Ms / Not Cited |
PA1-655 was used in immunocytochemistry to investigate the trafficking pathway of EtxB-peptide conjugates.
J Biol Chem. 2004 Dec 3;279(49):51315-22.
"Trafficking of exogenous peptides into proteasome-dependent major histocompatibility complex class I pathway following enterotoxin B subunit-mediated delivery." Author(s): Hearn AR, de Haan L, Pemberton AJ, Hirst TR, Rivett AJ Number of Citations: 1 (See PubMed article )
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| Immunohistochemistry | ||
| Species / Dilution | Summary | |
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Ms / Not Cited |
PA1-655 was used in immunohistochemistry to study the mechanism for the subcellular distributions of lysosome-associated membrane protein LAMP-2 splice variants
J Cell Biol. 1997 Jun 2;137(5):1161-9.
"Different steady state subcellular distributions of the three splice variants of lysosome-associated membrane protein LAMP-2 are determined largely by the COOH-terminal amino acid residue." Author(s): Gough NR, Fambrough DM Number of Citations: 13 (See PubMed article )
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| Western Blot | ||
| Species / Dilution | Summary | |
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Hu / Not Cited |
PA1-655 was used in western blot to investigate the anti-apoptotic mechanism of Hsp70 in human melanocytes
Carcinogenesis. 2007 Mar;28(3):537-44.
"Hsp70 protects against UVB induced apoptosis by preventing release of cathepsins and cytochrome c in human melanocytes." Author(s): Bivik C, Rosdahl I, Ollinger K Number of Citations: 1 (See PubMed article )
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Hu / 1:200 |
PA1-655 was used in western blot to identify and characterize beta hexosaminidases
Biosci Rep. 2008 Aug;28(4):229-37.
"Identification and characterization of mature beta-hexosaminidases associated with human placenta lysosomal membrane." Author(s): Magini A, Mencarelli S, Tancini B, Ciccarone V, Urbanelli L, Hasilik A, Emiliani C Number of Citations: 1 (See PubMed article )
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Ms / 0 |
PA1-655 was used in western blot to study the role of mitochondria and lysosomes in calpain-mediated epithelial-cell death during mammary gland involution
Cell Death Differ. 2012 Sep;19(9):1536-48.
"Calpains mediate epithelial-cell death during mammary gland involution: mitochondria and lysosomal destabilization." Author(s): Arnandis T, Ferrer-Vicens I, García-Trevijano ER, Miralles VJ, García C, Torres L, Viña JR, Zaragozá R Number of Citations: 0 (See PubMed article )
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Rt / Not Cited |
PA1-655 was used in western blot to identify membrane-associated RING-CH (MARCH) and its binding to syntaxin 6.
Mol Biol Cell. 2005 Apr;16(4):1696-710.
"MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking." Author(s): Nakamura N, Fukuda H, Kato A, Hirose S Number of Citations: 1 (See PubMed article )
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Rt / Not Cited |
PA1-655 was used in western blot to investigate the influence of chronic alcohol consumption on neuronal endocytosis
Toxicol Sci. 2010 May;115(1):202-13.
"Endocytosis in cultured neurons is altered by chronic alcohol exposure." Author(s): Marín MP, Esteban-Pretel G, Ponsoda X, Romero AM, Ballestín R, López C, Megías L, Timoneda J, Molowny A, Canales JJ, Renau-Piqueras J Number of Citations: 0 (See PubMed article )
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(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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LAMP2 Antibodies > LAMP2 Polyclonal Antibody PA1-655
