Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
100 ul of Whole serum with no preservatives.
Applications
Dilution *
Immunofluorescence (IF)
Assay dependent
Immunocytochemistry (ICC)
1:500
Western Blot (WB)
1:2,000
Immunoprecipitation (IP)
1:200
In vitro Assay (IV)
Assay-Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA1-46262 reacts with DJ-1 in human and murine samples. This antibody is predicted to react with rat, bovine, Zebra fish, Golden Syrian hamster, and chicken based on 100% sequence homology.
PA1-46262 has been successfully used in Western Blot, Immunocytochemistry/Immunofluorescence, In vitro assay and Immunoprecipitation applications.
The PA1-46262 immunogen is a synthetic peptide containing the C-terminus (within residues 150-189) of human Park7(DJ-1).
General Information
DJ-1 is one of several genes thought to cause autosomal-recessive early-onset parkinsonism.
Product Images
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DJ-1 Antibody (PA1-46262) in WB
Western Blot with anti-DJ-1 Polyclonal Antibody (PA1-46262)
Cells were transfected with the pCMV6-ENTRY control (lane 1) or pCMV6-ENTRY PARK7 cDNA (lane 2) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARK7.