CYLD Antibody
CYLD Polyclonal Antibody for ICC, IF, Western blot
Synonyms:
CDMT, CYLD1, CYLDI, EAC, FLJ20180, FLJ31664, FLJ78684, HSPC057, KIAA0849, MFT, MFT1, SBS, TEM, USPL2
Entrez Gene ID:
UniProt ID:
(Hu) Q9NQC7, (Ms) Q80TQ2
| Details | |
| Host / Isotype: | Rabbit / IgG |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Mouse (Ms) Canine (Ca) Non-human primate (Nhp) Bovine (Bv) |
| Immunogen: | Synthetic peptide corresponding to residues D(32) K Q T Q K L L K V P K G S(45) of human CYLD. |
| Ordering Information | ||||
| Pierce CYLD Antibody |
| Storage: | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Form: | 100 µg protein G purified antibody (0.5 mg/ml) in PBS containing 0.05% BSA and 0.05% sodium azide. |
| Applications | Dilution * |
| Western Blot (WB) | 2-4 µg/ml |
| Immunofluorescence (IF) | 5-10 ug/ml |
| Immunocytochemistry (ICC) | 5-10 ug/ml |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA1-41097 detects CYLD in bovine, chimpanzee, canine, human and murine samples. Predicted to react with rat. PA1-41097 has been successfully used in Western blot, immunofluorescence and immunocytochemistry applications. The PA1-41097 immunogen is a synthetic peptide corresponding to the N-terminal residues 32-45 of human CYLD. Positive Control: HeLa cell lysate. |
| General Information |
| CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. |
| Product Images |
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CYLD Antibody (PA1-41097) in IHC
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Immunohistochemistry with anti-CYLD Polyclonal Antibody (PA1-41097)
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CYLD Antibody (PA1-41097) in IHC
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Immunohistochemistry with anti-CYLD Polyclonal Antibody (PA1-41097)
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CYLD Antibody (PA1-41097) in WB
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Western Blot with anti-CYLD Polyclonal Antibody (PA1-41097)
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(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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CYLD Antibodies > CYLD Polyclonal Antibody PA1-41097
