Aggrecan G3 Antibody
Aggrecan G3 Polyclonal Antibody for Western blot
Synonyms:
AGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK
Entrez Gene ID:
UniProt ID:
(Hu) P16112.2, (Ms) Q61282, (Rt) P07897, (Bv) P13608, (Ca) Q28343
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Mouse (Ms) Rat (Rt) Canine (Ca) Bovine (Bv) |
| Immunogen: | Synthetic peptide corresponding to residues C D(2277) G H P M Q F E N W R P N Q P D N(2293) of human aggrecan. |
| Ordering Information | ||||
| Pierce Aggrecan G3 Antibody |
| Storage: | -20° C, Avoid Freeze/Thaw Cycles |
| Form: | 100 ug of affinity purified antibody (1mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution * |
| Western Blot (WB) | 2 ug/ml |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA1-1745 detects human, rat, mouse, bovine, and canine aggrecan G3. PA1-1745 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~350 kDa protein representing the aggrecan G3 domain on the full length core. The PA1-1745 immunogen is a synthetic peptide corresponding to residues C D(2277) G H P M Q F E N W R P N Q P D N(2293) of human aggrecan.. This antibody is also referred to as anti-G3. |
| General Information |
| Aggrecan is a member of large, aggregating proteoglycans (also including, versican, brevican and neurocan) found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMP’s) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. The G3 domain is a complicated region produced by alternative splicing of exons in post-transcriptional processes. The human G3 domain consists of three modules and articular cartilage contains a mixture of the alternatively spliced G3 modules. G3 has been shown to facilitate glycosaminoglycan (GAG) attachment and overall aggrecan processing. The G3 domain is central to aggrecan turnover and function in cartilage. |
| Product Images |
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Aggrecan G3 Antibody (PA1-1745) in WB
Western Blot with anti-Aggrecan G3 Polyclonal Antibody (PA1-1745)
Figure 1 illustrates Western blot detection of mouse chondrocyte cells using PA1-1745. The specificity of PA1-1745 is demonstrated in lane 1. Lane 1: mouse chondrocyte Lane 2: a lane of human cartilage proteoglycan blotted with anti-aggrecan G1 domain (general)
| PubMed References: |
| Western Blot | ||
| Species / Dilution | Summary | |
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Fs / Not Cited |
PA1-1745 was used in western blot to investigate the structural composition of proteoglycan in salmon nasal cartilage
Arch Biochem Biophys. 2011 Feb 1;506(1):58-65.
"Identification of proteoglycan from salmon nasal cartilage." Author(s): Kakizaki I, Tatara Y, Majima M, Kato Y, Endo M Number of Citations: 0 (See PubMed article  )
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Hu / 1:500 |
PA1-1745 was used in western blot to evaluate the effectiveness of various purification methods of aggrecan purification from human cartilage and synovial fluid
Matrix Biol. 2010 Jan;29(1):74-83.
"A comparison of different purification methods of aggrecan fragments from human articular cartilage and synovial fluid." Author(s): Struglics A, Larsson S Number of Citations: 0 (See PubMed article )
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Hu / 1:500 |
PA1-1745 was used in western blot to study the role oh calpain in the truncation of human aggrecan.
Biochem J. 2010 Sep 15;430(3):531-8.
"Calpain is involved in C-terminal truncation of human aggrecan." Author(s): Struglics A, Hansson M Number of Citations: 1 (See PubMed article )
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(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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Aggrecan G3 Antibodies > Aggrecan G3 Polyclonal Antibody PA1-1745
