100 ul of Antigen affinity chromatography purified antibody (1 mg/ml) in Tris glycine, 0.15M NaCl with 0.05% Sodium azide.
Western Blot (WB)
0.5 to 2.0 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA1-16996 detects TARDBP protein in human, nonhuman primate, murine, chicken, zebrafish and Cenopus laevis samples.
PA1-16996 has been successfully used in Western blot procedures. In Western blot, PA1-16996 is specific for the ~45 kDa TARDBP protein.
PA1-16996 immunogen is a synthetic peptide corresponding to residues 350-414 of the human TARDBP protein.
The HIV1 virus gives rise to acquired immunodeficiency syndrome (AIDS). HIV1 contains an RNA genome which produces chromosomally integrated DNA during cellular replication. Gene activation of HIV1 by the transactivator Tat requires an RNA regulatory element TAR which is located downstream of the transcription initiation site. TARDBP is a transcriptional repressor that binds to the chromosomally integrated TAR DNA, preventing HIV1 expression. Additionally, this protein regulates alternative splicing of the CTFR gene, a similar pseudogene on chromosoome 20. TARDBP has also been recently been shown to abnormally accumulate in the post-mortem brain tissue of individuals diagnosed with ALS (Amyotrophic Lateral Sclerosis) and FTD (Frontal Temporal Dementia). This long-awaited conncection between these two diseases provides an explanation for the observed clinical overlap.
TARDBP Antibody (PA1-16996) in WB
Western Blot with anti-TARDBP Polyclonal Antibody (PA1-16996)
cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY TARDBP cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TARDBP.