HIF Prolylhydroxylase 2 Antibody
HIF Prolylhydroxylase 2 Polyclonal Antibody for FACS, IF, IHC (P), Western blot
Synonyms:
C1ORF12, Chromosome 1 Open Reading Frame 12, EGL 9 homolog of C. elegans 1, EGLN 1 EGLN1, HIF Prolyl Hydroxylase 2, HIF-P4H 2, HIFPH2, P4H2, Prolyl Hy
Entrez Gene ID:
UniProt ID:
(Hu) Q9GZT9
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) |
| Immunogen: | A synthetic peptide made to an N-terminal sequence of the human EGL9 homolog 1 (between residues 1-50). [LocusLink ID 54583] |
| Ordering Information | ||||
| Pierce HIF Prolylhydroxylase 2 Antibody |
| Storage: | 4° C, do not freeze |
| Form: | 100 ul of Antigen affinity chromatography purified antibody (1 mg/ml) in Tris citrate/phosphate, pH 7-8, with 0.09% Sodium azide. |
| Applications | Dilution * |
| Immunofluorescence (IF) | 1:50 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay dependent |
| Flow Cytometry (FACS) | 3 µg/ml |
| Western Blot (WB) | 1:500-1:2500 |
| Immunocytochemistry (ICC) | 1:50 |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA1-16524 reacts with HIF Prolylhydroxylase 2 in human samples. This antibody does not react with mouse samples. PA1-16524 has been successfully used in Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Immunohistochemistry-Paraffin applications. The PA1-16524 immunogen corresponds to a region between residues 1 and 50 of human PHD2/HIF Prolyl Hydroxylase 2 using the numbering given in entry NP_0713341 (GeneID 54583). |
| General Information |
| HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degredation via the von Hippel-Lindau ubiquitylation complex. |
| Product Images |
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HIF Prolylhydroxylase 2 Antibody (PA1-16524) in WB
Western Blot with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524)
Western Blot analysis of human PHD2, using PA1-16524. Samples: Recombinant FLAG-His-PHD1, PHD2 or PHD3 (10ng/lane) and whole cell lysate from HeLa cells. -
HIF Prolylhydroxylase 2 Antibody (PA1-16524) in WB
Western Blot with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524)
Western Blot analysis of HeLa lysates using PA1-16524. -
HIF Prolylhydroxylase 2 Antibody (PA1-16524) in FACS
Flow Cytometry with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524)
Flow cytometric detection of PHD2. 1 million Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/ml anti-PHD2 PA1-16524 in a 150 ul reaction. Isotype control (black), anti-MLL1 (red). -
HIF Prolylhydroxylase 2 Antibody (PA1-16524) in ICC
Immunocytochemistry with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524)
Detection of HIF Prolyl Hydroxylase 2 (Green) in Hela cells using PA1-16524 at a dilution of 1:50. Nuclei (Blue) are counterstainde using Hoechst. -
HIF Prolylhydroxylase 2 Antibody (PA1-16524) in IHC
Immunohistochemistry with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524)
Immunohistochemical staining of Vascular Endothelium 40X in Lung using PA1-16524.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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HIF Prolylhydroxylase 2 Antibodies > HIF Prolylhydroxylase 2 Polyclonal Antibody PA1-16524
