100 ul of Antigen affinity chromatography purified antibody (1 mg/ml) in Tris citrate/phosphate, pH 7-8, with 0.09% Sodium azide.
Immunohistochemistry (Paraffin) (IHC (P))
Flow Cytometry (FACS)
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA1-16524 reacts with HIF Prolylhydroxylase 2 in human samples. This antibody does not react with mouse samples.
PA1-16524 has been successfully used in Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Immunohistochemistry-Paraffin applications.
The PA1-16524 immunogen corresponds to a region between residues 1 and 50 of human PHD2/HIF Prolyl Hydroxylase 2 using the numbering given in entry NP_0713341 (GeneID 54583).
HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degredation via the von Hippel-Lindau ubiquitylation complex.
HIF Prolylhydroxylase 2 Antibody (PA1-16524) in WB
Western Blot with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524)