HIF-2 alpha Antibody
HIF-2 alpha Polyclonal Antibody for ChIP, ELISA, FACS, ICC, IF, IHC (P), IP, Western blot
Synonyms:
Hypoxia inducible Factor 2 alpha, MOP2, Endothelial pas domain protein 1, Member of pas superfamily 2
Entrez Gene ID:
UniProt ID:
(Hu) Q99814, (Ms) P97481, (Rt) Q9JHS1
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Fish (Fs) Human (Hu) Mouse (Ms) Rat (Rt) |
| Immunogen: | A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein. |
| Ordering Information | ||||
| Pierce HIF-2 alpha Antibody |
| Storage: | 4° C, do not freeze |
| Form: | 100 ul of Antigen affinity chromatography purified antibody (2 mg/ml) in Tris glycine, 0.15M NaCl with 0.05% Sodium azide. |
| Applications | Dilution * |
| Western Blot (WB) | 1:200 - 1:1000 |
| Immunoprecipitation (IP) | 5 µl/mg lysate |
| ChIP assay (ChIP) | Assay Dependent |
| Immunocytochemistry (ICC) | 1:100 |
| ELISA (ELISA) | Assay Dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunofluorescence (IF) | 1:100 |
| Flow Cytometry (FACS) | Assay Dependent |
| Flow Cytometry (FACS) | Assay-Dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100 |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA1-16510 reacts with HIF-2 alpha in murine, rat, fish and human samples. PA1-16510 has been successfully used in Western Blot, Chromatin Immunoprecipitation, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Immunoprecipitation applications. The PA1-16510 immunogen is a peptide derived from the C-terminus of mouse/human HIF-2 alpha protein. |
| General Information |
| Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells. |
| Product Images |
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HIF-2 alpha Antibody (PA1-16510) in WB
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Western Blot with anti-HIF-2 alpha Polyclonal Antibody (PA1-16510)
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HIF-2 alpha Antibody (PA1-16510) in WB
Western Blot with anti-HIF-2 alpha Polyclonal Antibody (PA1-16510)
Western blot analysis of HIF-2 alpha on Lane 1, Cobalt chloride treated COS7 nuclear extracts and Lane 2, Untreated COS7 nuclear extracts using PA1-16510. -
HIF-2 alpha Antibody (PA1-16510) in WB
Western Blot with anti-HIF-2 alpha Polyclonal Antibody (PA1-16510)
Western Blot analysis on normoxic and hypoxic nuclear rat cell lysates using PA1-16510, anti-HIF-2 alpha. -
HIF-2 alpha Antibody (PA1-16510) in ICC
Immunocytochemistry with anti-HIF-2 alpha Polyclonal Antibody (PA1-16510)
Detection of HIF-2 Alpha (Green) in RCC4 cells using PA1-16510. Nuclei (Blue) are counterstained with Hoechst 33258. -
HIF-2 alpha Antibody (PA1-16510) in IHC
Immunohistochemistry with anti-HIF-2 alpha Polyclonal Antibody (PA1-16510)
Hif-2 alpha immunoreactivity in human cardiac myocytes stained with PA1-16510.
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(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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HIF-2 alpha Antibodies > HIF-2 alpha Polyclonal Antibody PA1-16510
