Cyclophilin B Antibody
Cyclophilin B Polyclonal Antibody for Western blot
Synonyms:
Cyp B, Peptidyl-prolyl cis-trans isomerase B, EC 5.2.1.8, PPIase, Rotamase, S-cyclophilin, SCYLP, CYP-S1
Entrez Gene ID:
UniProt ID:
(Hu) P23284, (Ms) P24369, (Rt) P24368
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Mouse (Ms) Rat (Rt) Canine (Ca) |
| Immunogen: | Synthetic peptide corresponding to residues C(194) G K I E V E K P F A I A K E(208) of human CyPB. |
| Ordering Information | ||||
| Pierce Cyclophilin B Antibody |
| Storage: | -20° C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µg of epitope affinity purified IgG (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution * |
| Western Blot (WB) | 0.5 µg/ml |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA1-027A detects cyclophilin B (CyPB) in mouse, rat, canine, and human tissues. PA1-027A has been sucessfully used in Western blot procedures, and previous lots have been used in immunocytochemistry. By Western blot, this antibody detects a ~19 kDa protein representing CyPB from rat liver extract. The PA1-027A immunogen is a synthetic peptide corresponding to residues C(194) G K I E V E K P F A I A K E(208) of human CyPB. This sequence is almost completely conserved between human, mouse, rat, bovine, and chicken. PA1-027A immunizing peptide (Cat. # PEP-058) is available for use in neutralization and control experiments. |
| General Information |
| Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins which bind FK506 are termed FK506 Binding Proteins (FKBP's) and those which bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation. Thus, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. |
| Product Images |
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Cyclophilin B Antibody (PA1-027A) in WB
Western Blot with anti-Cyclophilin B Polyclonal Antibody (PA1-027A)
Western blot analysis of Cyclophilin B in Rat Liver lysate (25 ug) using anti-Cyclophilin B antibody, PA1-027A at a dilution of 1:2000 (lane 2), 1:1000 (lane 3), 1:2000 (lane 4) and 1:4000 (lane 5).
| PubMed References: |
| Immunohistochemistry | ||
| Species / Dilution | Summary | |
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Hu / Not Cited |
PA1-027A was used in immunohistochemistry to study the mechanism for the cytoplasmic accumulation of extracellular matrix proteins and chaperones associated in pseudoachondroplasia
Matrix Biol. 2001 Nov;20(7):439-50.
"Selective intracellular retention of extracellular matrix proteins and chaperones associated with pseudoachondroplasia." Author(s): Vranka J, Mokashi A, Keene DR, Tufa S, Corson G, Sussman M, Horton WA, Maddox K, Sakai L, Bächinger HP Number of Citations: 17 (See PubMed article  )
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| Immunoprecipitation | ||
| Species / Dilution | Summary | |
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Hu / Not Cited |
PA1-027A was used in immunoprecipitation to study the role of cyclophin B in kidney function and the mechanisms of cyclsporin A toxicity.
PLoS One. 2010 Nov 10;5(11):e13930.
"Cyclophilin B interacts with sodium-potassium ATPase and is required for pump activity in proximal tubule cells of the kidney." Author(s): Suñé G, Sarró E, Puigmulé M, López-Hellín J, Zufferey M, Pertel T, Luban J, Meseguer A Number of Citations: 1 (See PubMed article )
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| Western Blot | ||
| Species / Dilution | Summary | |
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Hu / 1:1,000 |
PA1-027A was used in western blot to study the effects of cyclosporin A and PSC833 on the activity and expression of the sodium/calcium exchanger NCX1
J Biol Chem. 2002 Jan 25;277(4):2505-10.
"Transport activity and surface expression of the Na+-Ca2+ exchanger NCX1 are inhibited by the immunosuppressive agent cyclosporin A and by the nonimmunosuppressive agent PSC833." Author(s): Kimchi-Sarfaty C, Kasir J, Ambudkar SV, Rahamimoff H Number of Citations: 1 (See PubMed article )
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Hu / Not Cited |
PA1-027A was used in western blot to study the effect of lentiviral short-hairpin RNAs delivery on interferon pathway activation
Nucleic Acids Res. 2009 Oct;37(19):6587-99.
"Short-hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation." Author(s): Kenworthy R, Lambert D, Yang F, Wang N, Chen Z, Zhu H, Zhu F, Liu C, Li K, Tang H Number of Citations: 5 (See PubMed article )
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Hu / Not Cited |
PA1-027A was used in western blot to investigate the role of acyl-CoA dehydrogenase 9 in oxidative phosphorylation complex I formation
Cell Metab. 2010 Sep 8;12(3):283-94.
"Acyl-CoA dehydrogenase 9 is required for the biogenesis of oxidative phosphorylation complex I." Author(s): Nouws J, Nijtmans L, Houten SM, van den Brand M, Huynen M, Venselaar H, Hoefs S, Gloerich J, Kronick J, Hutchin T, Willems P, Rodenburg R, Wanders R, van den Heuvel L, Smeitink J, Vogel RO Number of Citations: 9 (See PubMed article )
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Hu / Not Cited |
PA1-027A was used in western blot to investigate the effect of calcineurin signaling on human islet beta-cell survival
J Biol Chem. 2010 Dec 17;285(51):40050-9.
"Calcineurin signaling regulates human islet {beta}-cell survival." Author(s): Soleimanpour SA, Crutchlow MF, Ferrari AM, Raum JC, Groff DN, Rankin MM, Liu C, De León DD, Naji A, Kushner JA, Stoffers DA Number of Citations: 4 (See PubMed article )
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Hu / 0 |
PA1-027A was used in western blot to investigate the effect of PPIB mutations on type I procollagen chain association and osteogenesis
Hum Mol Genet. 2011 Apr 15;20(8):1595-609.
"Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and result in perinatal lethal to moderate osteogenesis imperfecta phenotypes." Author(s): Pyott SM, Schwarze U, Christiansen HE, Pepin MG, Leistritz DF, Dineen R, Harris C, Burton BK, Angle B, Kim K, Sussman MD, Weis M, Eyre DR, Russell DW, McCarthy KJ, Steiner RD, Byers PH Number of Citations: 1 (See PubMed article )
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Hu / 1:20000 |
PA1-027A was used in western blot to characterize the expression of glutathione S-transferase in liver
Exp Physiol. 2011 Aug;96(8):790-800.
"Liver glutathione S-transferase expression is decreased by 3,5,3-triiodothyronine in hypothyroid but not in euthyroid mice." Author(s): Faustino LC, Pires RM, Lima AC, Cordeiro A, Souza LL, Ortiga-Carvalho TM Number of Citations: 0 (See PubMed article )
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Ms / Not Cited |
PA1-027A was used in western blot to investigate the effect of RANK signal inhibition on osteoclast function
Pharm Res. 2010 Jul;27(7):1273-84.
"siRNA knock-down of RANK signaling to control osteoclast-mediated bone resorption." Author(s): Wang Y, Grainger DW Number of Citations: 3 (See PubMed article )
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Ms / Not Cited |
PA1-027A was used in western blot to investigate the therapeutic benefit of mTOR inhibition in the amelioration of fibrotic encapsulation
J Control Release. 2010 Nov 1;147(3):400-7.
"Device-based local delivery of siRNA against mammalian target of rapamycin (mTOR) in a murine subcutaneous implant model to inhibit fibrous encapsulation." Author(s): Takahashi H, Wang Y, Grainger DW Number of Citations: 1 (See PubMed article )
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Ms / 0 |
PA1-027A was used in western blot to evaluate a small interfering RNA against farnesyl pyrophosphate synthase
Mol Pharm. 2011 Aug 1;8(4):1016-24.
"Small interfering RNA knocks down the molecular target of alendronate, farnesyl pyrophosphate synthase, in osteoclast and osteoblast cultures." Author(s): Wang Y, Panasiuk A, Grainger DW Number of Citations: 1 (See PubMed article )
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Ms / 1:3000 |
PA1-027A was used in western blot to study the ability of blocking leptin after weaning to substantially reverse the the effects of neonatal hyperleptinaemia.
Horm Metab Res. 2011 Mar;43(3):171-7.
"Blocking leptin action one week after weaning reverts most of the programming caused by neonatal hyperleptinemia in the adult rat." Author(s): Trotta PA, Moura EG, Franco JG, Lima NS, de Oliveira E, Cordeiro A, Souza LL, Oliveira KJ, Lisboa PC, Pazos Moura CC, Passos MC Number of Citations: 0 (See PubMed article )
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Ms / 1:200 |
PA1-027A was used in western blot to study the role of myosin II activity in focal adhesion using proteomics
Nat Cell Biol. 2011 Apr;13(4):383-93.
"Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation." Author(s): Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM Number of Citations: 21 (See PubMed article )
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Rt / 1:100000 |
PA1-027A was used in western blot to examine the role of antioxidant enzymes in rat retinal ischemia-reperfusion injury and recovery
Metabolism. 2006 Jul;55(7):892-8.
"Expression of antioxidant enzymes in rat retinal ischemia followed by reperfusion." Author(s): Agardh CD, Gustavsson C, Hagert P, Nilsson M, Agardh E Number of Citations: 2 (See PubMed article )
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Rt / 1:15000 |
PA1-027A was used in western blot to investigate the effect of protein kinase C on serine racemase and D-serine availability
J Neurochem. 2011 Jan;116(2):281-90.
"Protein kinase C activity regulates D-serine availability in the brain." Author(s): Vargas-Lopes C, Madeira C, Kahn SA, Albino do Couto I, Bado P, Houzel JC, De Miranda J, de Freitas MS, Ferreira ST, Panizzutti R Number of Citations: 1 (See PubMed article )
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Rt / 1:10000 |
PA1-027A was used in western blot to investigate the protective effect of D1/D5 receptor activation on synapse function
J Biol Chem. 2011 Feb 4;286(5):3270-6.
"Activation of D1/D5 dopamine receptors protects neurons from synapse dysfunction induced by amyloid-beta oligomers." Author(s): Jürgensen S, Antonio LL, Mussi GE, Brito-Moreira J, Bomfim TR, De Felice FG, Garrido-Sanabria ER, Cavalheiro ÉA, Ferreira ST Number of Citations: 2 (See PubMed article )
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Rt / 1:50000 |
PA1-027A was used in western blot to investigate the role of thyroid hormone signaling in the hypolipidemic effect of n-3 polyunsaturated fatty acidsn-3
J Endocrinol. 2011 Oct;211(1):65-72.
"Thyroid hormone contributes to the hypolipidemic effect of polyunsaturated fatty acids from fish oil: in vivo evidence for cross talking mechanisms." Author(s): Souza LL, Cordeiro A, Oliveira LS, de Paula GS, Faustino LC, Ortiga-Carvalho TM, Oliveira KJ, Pazos-Moura CC Number of Citations: 0 (See PubMed article )
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(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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Cyclophilin B Antibodies > Cyclophilin B Polyclonal Antibody PA1-027A
