IL-13 Antibody
IL-13 Polyclonal Antibody for ELISA, Western blot
| Details | |
| Host / Isotype: | Rabbit / IgG |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) |
| Immunogen: | Recombinant human IL-13 |
| Ordering Information | ||||
| Pierce IL-13 Antibody |
| Storage: | -20°C |
| Form: | 1 mg of purified antibody from rabbit serum by Protein A affinity chromatography; in PBS, preservative- and carrier free |
| Applications | Dilution * |
| ELISA (ELISA) | Assay Dependent |
| Western Blot (WB) | Assay Dependent |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| P130E targets IL-13 in ELISA, and WB applications and shows reactivity with Human samples. The P130E immunogen is recombinant human IL-13. P130E detects IL-13 which has a predicted molecular weight of approximately 13 kDa. The P130E IL13 polyclonal antibody has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M130B (biotinylated conjugate of clone 5A2) Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection. Antibody P130E and biotinylated antibody M130B (clone 5A2) have successfully been used in combination with recombinant IL13 protein SIL13 in ELISA applications. |
| General Information |
| This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. |
| PubMed References: |
| ELISA | ||
| Species / Dilution | Summary | |
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Hu / 1:2,000 |
P130E was used in ELISA to investigate the role of plasmodium vivax duffy binding protein
J Immunol. 2002 Sep 15;169(6):3200-7.
"Age-dependent cellular immune responses to Plasmodium vivax Duffy binding protein in humans." Author(s): Xainli J, Baisor M, Kastens W, Bockarie M, Adams JH, King CL Number of Citations: 7 (See PubMed article  )
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Hu / Not Cited |
P130E was used in ELISA to characterize the prenatal immune responses against Plasmodium falciparum erythrocyte invasion ligands
Infect Immun. 2005 Jun;73(6):3462-70.
"Distinct Th1- and Th2-Type prenatal cytokine responses to Plasmodium falciparum erythrocyte invasion ligands." Author(s): Malhotra I, Mungai P, Muchiri E, Ouma J, Sharma S, Kazura JW, King CL Number of Citations: 1 (See PubMed article )
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Hu / Not Cited |
P130E was used in ELISA to study the interleukin 13 production in infants with different pertussis-tetanus-diphtheria-poliomyelitis vaccines
Vaccine. 2007 Jan 4;25(2):391-8.
"Modulation of the infant immune responses by the first pertussis vaccine administrations." Author(s): Mascart F, Hainaut M, Peltier A, Verscheure V, Levy J, Locht C Number of Citations: 1 (See PubMed article )
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Hu / Not Cited |
P130E was used in ELISA to study the effect of the prenatal malaria exposure on immune tolerance
PLoS Med. 2009 Jul;6(7):e1000116.
"Can prenatal malaria exposure produce an immune tolerant phenotype? A prospective birth cohort study in Kenya." Author(s): Malhotra I, Dent A, Mungai P, Wamachi A, Ouma JH, Narum DL, Muchiri E, Tisch DJ, King CL Number of Citations: 1 (See PubMed article )
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Hu / Not Cited |
P130E was used in ELISA to study the role of HLA-DQ molecules in Th-cell responses
Lab Invest. 2010 Dec;90(12):1747-56.
"Fibroblasts stimulated via HLA-II molecules produce prostaglandin E? and regulate cytokine production from helper T cells." Author(s): Kato-Kogoe N, Ohyama H, Nishimura F, Meguro M, Yoshizawa S, Okada Y, Nakasho K, Yamanegi K, Yamada N, Hata M, Higashi T, Terada N, Matsushita S Number of Citations: 1 (See PubMed article )
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(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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IL-13 Antibodies > IL-13 Polyclonal Antibody P130E
