Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
100 ul of ascitic fluid containing 0.03% sodium azide.
Western Blot (WB)
1/500 - 1/2000
1/200 - 1/1000
Flow Cytometry (FACS)
1/200 - 1/400
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA5-15918 targets G6PD in FACS, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15918 immunogen is purified recombinant fragment of human G6PD expressed in E. Coli. .
MA5-15918 detects G6PD which has a predicted molecular weight of approximately 59kDa.
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.
G6PD Antibody (MA5-15918) in IHC
Immunohistochemistry with anti-G6PD Monoclonal Antibody [5E12] (MA5-15918)