Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
100 ul of ascitic fluid containing 0.03% sodium azide.
1/200 - 1/1000
Flow Cytometry (FACS)
1/200 - 1/400
Western Blot (WB)
1/500 - 1/2000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA5-15883 targets IRAK4 in FACS, IHC, and WB applications and shows reactivity with Human, Murine, and Non-human primate samples.
The MA5-15883 immunogen is purified recombinant fragment of human IRAK4 expressed in E. Coli. .
MA5-15883 detects IRAK4 which has a predicted molecular weight of approximately 52kDa.
This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene.
IRAK4 Antibody (MA5-15883) in IHC
Immunohistochemistry with anti-IRAK4 Monoclonal Antibody [2H9] (MA5-15883)