Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
100 ul of ascitic fluid containing 0.03% sodium azide.
Western Blot (WB)
1/500 - 1/2000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA5-15878 targets GCK in WB applications and shows reactivity with Human samples.
The MA5-15878 immunogen is purified recombinant fragment of human GCK expressed in E. Coli. .
MA5-15878 detects GCK which has a predicted molecular weight of approximately 52kDa.
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
GCK Antibody (MA5-15878) in WB
Western Blot with anti-GCK Monoclonal Antibody [4G6] (MA5-15878)