HK1 Antibody (7A7)

HK1 Monoclonal Antibody for IF, IHC, Western blot

>> See 7 other antibodies for HK1 (HKI; HXK1; HK1-ta; HK1-tb; HK1-tc; HK1)
Synonyms:
HKI; HXK1; HK1-ta; HK1-tb; HK1-tc; HK1
Entrez Gene ID:
(Hu) 3098, (Ms) 15275, (Rt) 25058
UniProt ID:
(Hu) P19367, (Rt) P05708
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 7A7
Species Reactivity: Human (Hu) Mouse (Ms) Rat (Rt)
Immunogen: Purified recombinant fragment of human HK1 expressed in E. Coli.
Ordering Information
Pierce HK1 Antibody (7A7)
Product #
MA5-15675
Size
100 ul
Price
$299.00
Purchase
Add HK1 Antibody (7A7) to your cart.
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: 100 ul of ascitic fluid containing 0.03% sodium azide.


Applications Dilution *
Western Blot (WB) 1/500 - 1/2000
Immunofluorescence (IF) 1/200 - 1/1000
Immunohistochemistry (IHC) 1/200 - 1/1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA5-15675 targets HK1 in IF, IHC, and WB applications and shows reactivity with Human, Murine, and Rat samples.

The MA5-15675 immunogen is purified recombinant fragment of human HK1 expressed in E. Coli.

MA5-15675 detects HK1 which has a predicted molecular weight of approximately 102kDa.
General Information
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.
Product Images
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  • HK1 Antibody (MA5-15675) in ICC
    Immunocytochemistry with anti-HK1 Monoclonal Antibody [7A7] (MA5-15675)

    Immunocytochemistry with anti-HK1 Monoclonal Antibody [7A7] (MA5-15675)

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    Immunofluorescence analysis of NIH3T3 cells using HK1 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
     
  • HK1 Antibody (MA5-15675) in IHC
    Immunohistochemistry with anti-HK1 Monoclonal Antibody [7A7] (MA5-15675)

    Immunohistochemistry with anti-HK1 Monoclonal Antibody [7A7] (MA5-15675)

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    Immunohistochemical analysis of paraffin-embedded human salivary gland tissues (left) and kidney tissues (right) using HK1 antibody with DAB staining.
     
  • HK1 Antibody (MA5-15675) in WB
    Western Blot with anti-HK1 Monoclonal Antibody [7A7] (MA5-15675)

    Western Blot with anti-HK1 Monoclonal Antibody [7A7] (MA5-15675)

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    Western blot analysis using HK1 antibody against Jurket (1), Hela (2), HepG2 (3), MCF-7 (4) and PC-12 (5) cell lysate.
     
 
 
References:
Cell. 2005 Sep 23;122(6):957-68.
J Biomed Sci. 2007 Mar;14(2):195-202.
J Neural Transm. 2009 Mar;116(3):275-89.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific