Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
100 ul of ascitic fluid containing 0.03% sodium azide.
Western Blot (WB)
1/500 - 1/2000
1/200 - 1/1000
1/200 - 1/1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA5-15675 targets HK1 in IF, IHC, and WB applications and shows reactivity with Human, Murine, and Rat samples.
The MA5-15675 immunogen is purified recombinant fragment of human HK1 expressed in E. Coli.
MA5-15675 detects HK1 which has a predicted molecular weight of approximately 102kDa.
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.
HK1 Antibody (MA5-15675) in ICC
Immunocytochemistry with anti-HK1 Monoclonal Antibody [7A7] (MA5-15675)