PTEN Antibody (1B8)

PTEN Monoclonal Antibody for FACS, ICC, Western blot

>> See 9 other antibodies for PTEN (BZS; MHAM; TEP1; MMAC1; PTEN1; 10q23del; MGC11227; PTEN)
Synonyms:
BZS; MHAM; TEP1; MMAC1; PTEN1; 10q23del; MGC11227; PTEN
Entrez Gene ID:
(Hu) 5728, (Ms) 19211
UniProt ID:
(Hu) P60484, (Ms) O08586
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 1B8
Species Reactivity: Human (Hu) Mouse (Ms)
Immunogen: Purified recombinant fragment of PTEN expressed in E. Coli.
Ordering Information
Pierce PTEN Antibody (1B8)
Product #
MA5-15560
Size
100 ul
Price
$299.00
Purchase
Add PTEN Antibody (1B8) to your cart.
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: 100 ul of antibody are purified by protein G affinity chromatography in PBS containing 50% glycerol and 0.03% sodium azide.


Applications Dilution *
Western Blot (WB) 1/500 - 1/2000
Flow Cytometry (FACS) 1/200 - 1/400
Immunocytochemistry (ICC) 1:200 - 1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA5-15560 targets PTEN in ICC, FACS, and WB applications and shows reactivity with Human and Murine samples.

The MA5-15560 immunogen is purified recombinant fragment of PTEN expressed in E. Coli.

MA5-15560 detects PTEN which has a predicted molecular weight of approximately 54kDa.
General Information
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.
Product Images
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  • PTEN Antibody (MA5-15560) in FACS
    Flow Cytometry with anti-PTEN Monoclonal Antibody [1B8] (MA5-15560)

    Flow Cytometry with anti-PTEN Monoclonal Antibody [1B8] (MA5-15560)

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    Flow cytometric analysis of Hela cells using PTEN antibody (right) and negative control (left).
     
  • PTEN Antibody (MA5-15560) in IF
    Immunofluorescence with anti-PTEN Monoclonal Antibody [1B8] (MA5-15560)

    Immunofluorescence with anti-PTEN Monoclonal Antibody [1B8] (MA5-15560)

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    Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using PTEN antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
     
  • PTEN Antibody (MA5-15560) in WB
    Western Blot with anti-PTEN Monoclonal Antibody [1B8] (MA5-15560)

    Western Blot with anti-PTEN Monoclonal Antibody [1B8] (MA5-15560)

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    Western blot analysis using PTEN antibody against Hela (1) and NIH/3T3 (2) cell lysate.
     
 
 
References:
Nat Genet. 1998 Aug;19(4):348-55.
Oncogene. 1999 May 20;18(20):3181-5.
Eur J Immunol. 2002 Apr;32(4):1196-204.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific