Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
100 ul of antibody are purified by protein G affinity chromatography in PBS containing 50% glycerol and 0.03% sodium azide.
Western Blot (WB)
1/500 - 1/2000
Flow Cytometry (FACS)
1/200 - 1/400
1:200 - 1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA5-15560 targets PTEN in ICC, FACS, and WB applications and shows reactivity with Human and Murine samples.
The MA5-15560 immunogen is purified recombinant fragment of PTEN expressed in E. Coli.
MA5-15560 detects PTEN which has a predicted molecular weight of approximately 54kDa.
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.
PTEN Antibody (MA5-15560) in FACS
Flow Cytometry with anti-PTEN Monoclonal Antibody [1B8] (MA5-15560)