100 ul of antibody in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
Immunohistochemistry (Frozen) (IHC (F))
Immunohistochemistry (Paraffin) (IHC (P))
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA5-15143 targets PDGF Receptor beta in ICC, IHC (F), IHC (P), IP, and WB applications and shows reactivity with Human, Murine, and Rat samples.
The MA5-15143 immunogen is gST fusion protein containing a carboxy-terminal fragment of human PDGF receptor-beta.
MA5-15143 detects PDGF Receptor beta which has a predicted molecular weight of approximately 190 kDa.
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.
PDGF Receptor beta Antibody (MA5-15143) in IF
Immunofluorescence with anti-PDGF Receptor beta Monoclonal Antibody [G.290.3] (MA5-15143)
Immunofluorescent analysis of PDGF Receptor beta in NIH/3T3 cells, PDGF-treated, using a PDGF Receptor beta monoclonal antibody (Product # MA5-15143) (green). DNA is labeled using a fluorescent blue dye.