100 ul of antibody in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
Flow Cytometry (FACS)
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA5-14845 targets Oct-4A in FACS, ICC, and WB applications and shows reactivity with Human, and Murine samples.
The MA5-14845 immunogen is recombinant protein specific to the amino terminus of human Oct-4A.
MA5-14845 detects Oct-4A which has a predicted molecular weight of approximately 45 kDa.
This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Oct-4A Antibody (MA5-14845) in IF
Immunofluorescence with anti-Oct-4A Monoclonal Antibody [T.631.9] (MA5-14845)
Immunofluorescent analysis of Oct-4A in mouse embryonic stem cells growing on mouse embryonic fibroblast feeder cells, using an oct-4A monoclonal antibody (Product # MA5-14845) (green). Actin filaments are labeled with a fluorescent red phalloidin.