von Willebrand Factor Antibody (F8/86)

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von Willebrand Factor Monoclonal Antibody for IHC (P)

>> See 14 other antibodies for VWF (coagulation factor VIII VWF)

Synonyms:

coagulation factor VIII VWF

Entrez Gene ID:

(Hu) 7450

UniProt ID:

(Hu) P04275

Details

Host / Isotype:	Mouse / IgG1, kappa
Class:	Monoclonal
Type:	Antibody
Clone:	F8/86
Species Reactivity:	Human (Hu)
Immunogen:	Partially purified von Willebrand factor from human placenta

Ordering Information
Pierce von Willebrand Factor Antibody (F8/86)

Product #

MA5-14029

Size

500 ul

Price

$266.00

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Storage:	4° C, do not freeze
Form:	500 ul of antibody purified from the ascites fluid by Protein G chromatography (200 ug/ml). Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Applications	Dilution *

Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:100
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Product Specific Information

MA5-14029 targets von Willebrand Factor in IHC (P) applications and shows reactivity with Human samples.

The MA5-14029 immunogen is partially purified von Willebrand factor from human placenta.

General Information

Factor VIII related antigen or von Willebrand factor is a multimeric glycoprotein, which binds to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. It is synthesized by endothelial cells and stored in the Weibel-Palade granules. It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII. von Willebrand factor is one of the most useful markers to identify endothelial (or megakaryocytic) lineage of neoplasms. As not all endothelial cells synthesize/ store) this molecule, about 30% of tumors of vascular origin fail to stain for factor VIII related antigen, regardless of whether they are benign or malignant. Staining for factor VIII related antigen has also been used to measure angiogenesis, an indicator of tumor recurrence.

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von Willebrand Factor Antibody (MA5-14029) in IHC

Immunohistochemistry with anti-von Willebrand Factor Monoclonal Antibody [F8/86] (MA5-14029)

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Formalin-fixed, paraffin-embedded human tonsil stained with Factor VIII antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of endothelial cells.

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PubMed References:

ICC (3)
IHC (6)
IP (1)
WB (1)

Immunocytochemistry
	Species / Dilution	Summary
	Hu / 1:64	MA5-14029 was used in immunocytochemistry to develop a method for isolation and characterization of human gingival microvascular endothelial cells J Periodontal Res. 2008 Apr;43(2):246-54. "Isolation and characterization of human gingival microvascular endothelial cells." Author(s): DeCarlo AA, Cohen JA, Aguado A, Glenn B Number of Citations: 2 (See PubMed article )
	Hu / 1:150	MA5-14029 was used in immunocytochemistry to investigate the effect of fibronectin on macrophage differentiation of HL60 cells induced by phorbol 12-myristate 13-acetate Int J Hematol. 2009 Mar;89(2):167-72. "Fibronectin promotes the phorbol 12-myristate 13-acetate-induced macrophage differentiation in myeloid leukemia cells." Author(s): Esendagli G, Canpinar H, Yilmaz G, Kaymaz FF, Kansu E, Guc D Number of Citations: 1 (See PubMed article )
	Hu / Not Cited	MA5-14029 was used in immunocytochemistry and immunoprecipitation to investigate the mechanism of coagulation factor FXIII-A subcellular transport and secretion in immune cells Blood. 2010 Apr 1;115(13):2674-81. "Association of coagulation factor XIII-A with Golgi proteins within monocyte-macrophages: implications for subcellular trafficking and secretion." Author(s): Cordell PA, Kile BT, Standeven KF, Josefsson EC, Pease RJ, Grant PJ Number of Citations: 2 (See PubMed article )

Immunohistochemistry
	Species / Dilution	Summary
	Hu / Not Cited	MA5-14029 was used in immunohistochemistry to investigate the role of urokinase receptor in the pathogenesis of malignant pleural mesothelioma Am J Respir Cell Mol Biol. 2010 Jun;42(6):685-96. "The urokinase receptor supports tumorigenesis of human malignant pleural mesothelioma cells." Author(s): Tucker TA, Dean C, Komissarov AA, Koenig K, Mazar AP, Pendurthi U, Allen T, Idell S Number of Citations: 3 (See PubMed article )
	Hu / 1:50	MA5-14029 was used in immunohistochemistry to study the molecualr basis of lymphatic differentiation in classic Kaposi's sarcoma Pathol Oncol Res. 2011 Dec;17(4):843-51. "Lymphatic differentiation in classic Kaposi's sarcoma: patterns of D2-40 immunoexpression in the course of tumor progression." Author(s): Kandemir NO, Barut F, Gun BD, Keser SH, Karadayi N, Gun M, Ozdamar SO Number of Citations: 0 (See PubMed article )
	Hu / 0	MA5-14029 was used in immunohistochemistry to investigate the relationship between endothelium-coated tumor clusters and severity of liver cancer patients Cancer. 2011 Nov 1;117(21):4878-89. "Endothelium-coated tumor clusters are associated with poor prognosis and micrometastasis of hepatocellular carcinoma after resection." Author(s): Ding T, Xu J, Zhang Y, Guo RP, Wu WC, Zhang SD, Qian CN, Zheng L Number of Citations: 1 (See PubMed article )
	Hu / 0	MA5-14029 was used in immunohistochemistry to study the prognostic value of bone marrow microvessel density and angiogenic cytokines in patients undergoing autologous stem cell transplant Leuk Lymphoma. 2011 Jul;52(7):1281-9. "Prognostic value of bone marrow microvessel density and angiogenic cytokines in patients with multiple myeloma undergoing autologous stem cell transplant." Author(s): Sucak GT, Aki SZ, Yüzba?io?lu B, Akyürek N, Ya?ci M, Ba?riaçik U, Haznedar R Number of Citations: 0 (See PubMed article )
	Nhp / Not Cited	MA5-14029 was used in immunohistochemistry to detect the presence of lymphatic vessels in the corpus luteum Lymphat Res Biol. 2009;7(3):159-68. "Existence of the lymphatic system in the primate corpus luteum." Author(s): Xu F, Stouffer RL Number of Citations: 1 (See PubMed article )
	Rt / 0	MA5-14029 was used in immunohistochemistry to investigate the effect of pulsed acoustic cellular treatment on gene expression in muscle flaps J Trauma. 2010 Dec;69(6):1448-56. "Pulsed acoustic cellular treatment induces expression of proangiogenic factors and chemokines in muscle flaps." Author(s): Krokowicz L, Cwykiel J, Klimczak A, Mielniczuk M, Siemionow M Number of Citations: 1 (See PubMed article )

Immunoprecipitation
	Species / Dilution	Summary
	Hu / 10 ug/ml	MA5-14029 was used in immunocytochemistry and immunoprecipitation to investigate the mechanism of coagulation factor FXIII-A subcellular transport and secretion in immune cells Blood. 2010 Apr 1;115(13):2674-81. "Association of coagulation factor XIII-A with Golgi proteins within monocyte-macrophages: implications for subcellular trafficking and secretion." Author(s): Cordell PA, Kile BT, Standeven KF, Josefsson EC, Pease RJ, Grant PJ Number of Citations: 2 (See PubMed article )

Western Blot
	Species / Dilution	Summary
	Hu / 0	MA5-14029 was used in western blot to study the role of VEGF in regulating Notch1 and Dll4 expression in arterial endothelial cells Mol Cell Biol. 2003 Jan;23(1):14-25. "Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis." Author(s): Liu ZJ, Shirakawa T, Li Y, Soma A, Oka M, Dotto GP, Fairman RM, Velazquez OC, Herlyn M Number of Citations: 72 (See PubMed article )

(This product is for In Vitro experimental use only. Not for resale without express authorization.)