Surfactant Protein B (Precursor) Antibody, Biotin conjugate (SPB01 (1-2-A5-9))

Surfactant Protein B (Precursor) Monoclonal Antibody for IF, IHC (P)

>> See 3 other antibodies for SFTPB (18 kDa pulmonary-surfactant protein, 6 kDa protein,pulmonary surfactant-associated protein B, pulmonary surfactant-associated proteolipid SPL(Phe))

Synonyms:

18 kDa pulmonary-surfactant protein, 6 kDa protein,pulmonary surfactant-associated protein B, pulmonary surfactant-associated proteolipid SPL(Phe)

Entrez Gene ID:

(Hu) 6439, (Rt) 192155

UniProt ID:

(Hu) P07988, (Rt) P22355

Details

Host / Isotype:	Mouse / IgG2a, kappa
Class:	Monoclonal
Type:	Antibody
Clone:	SPB01 (1-2-A5-9)
Label:	Biotin
Species Reactivity:	Human (Hu) Rat (Rt)
Immunogen:	Recombinant pro-surfactant protein B

Ordering Information
Pierce Surfactant Protein B (Precursor) Antibody, Biotin conjugate (SPB01 (1-2-A5-9))

Product #

MA5-13972

Size

500 ul

Price

$397.00

Purchase

Bulk Order?

Storage:	4° C, do not freeze
Form:	500 ul of antibody purified from ascites fluid by Protein A chromatography (200 ug/ml). Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Applications	Dilution *

Immunofluorescence (IF)	Assay Dependent
Immunohistochemistry (Paraffin) (IHC (P))	2-4 µg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Product Specific Information

MA5-13972 targets Surfactant Protein B (Precursor) in IF and IHC (P) applications and shows reactivity with Human and Rat samples.

The MA5-13972 immunogen is recombinant pro-surfactant protein B.

General Information

Pulmonary surfactant is a complex mixture of phospholipids and proteins that is secreted from type II cells in alveoli and reduces the surface tension at the alveolar air-liquid interface, providing alveolar stability necessary for normal ventillation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C, and D. SP-A (28-36kDa) and SP-D (43kDa) are collagenous carbohydrate-binding proteins, whereas SP-B (8-9kDa) and SP-C (4kDa) are non-collagenous hydrophobic proteins. SP-B is expressed in pulmonary adenocarcinomas with acinar, papillary, bronchioloalveolar, and solid growth patterns. Squamous cell and large cell carcinomas of the lung and nonpulmonary adenocarcinomas do not express SP-B. ProSP-B is glycosylated in the Golgi apparatus and undergoes carboxy- and amino-terminal proteolysis by a cathepsin D-like protease.

Product Images

Filter :

Surfactant Protein B (Precursor) Antibody (MA5-13972) in IHC

Immunohistochemistry with anti-Surfactant Protein B (Precursor) Monoclonal Antibody [SPB01 (1-2-A5-9)], Biotin conjugate (MA5-13972)

View Product Details >>

Formalin-fixed, paraffin-embedded human lung stained with Prosurfactant Protein B antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of alveolar type-II epithelial cells.

... more >>
Surfactant Protein B (Precursor) Antibody (MA5-13972) in IHC

Immunohistochemistry with anti-Surfactant Protein B (Precursor) Monoclonal Antibody [SPB01 (1-2-A5-9)], Biotin conjugate (MA5-13972)

View Product Details >>
Surfactant Protein B (Precursor) Antibody (MA5-13972) in IHC

Immunohistochemistry with anti-Surfactant Protein B (Precursor) Monoclonal Antibody [SPB01 (1-2-A5-9)], Biotin conjugate (MA5-13972)

View Product Details >>

Formalin-fixed, paraffin-embedded rat lung stained with Prosurfactant Protein B antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of alveolar type-II cells.

... more >>

PubMed References:

IHC (3)

Immunohistochemistry
	Species / Dilution	Summary
	Hu / 1:50	MA5-13972 was used in immunohistochemistry to report a clinical case of basaloid adenocarcinoma Virchows Arch. 2007 Sep;451(3):729-36. "Basaloid adenocarcinoma. A new variant of pulmonary adenocarcinoma." Author(s): Marci V, Volante M, Cappia S, Righi L, Novello C, Scagliotti GV, Brambilla E, Papotti M Number of Citations: 0 (See PubMed article )
	Hu / 1:25	MA5-13972 was used in immunohistochemistry to examine the diagnostic significance of thyroid transcription factor-1 in ovarian and uterine epithelial neoplasia Int J Gynecol Pathol. 2009 Jan;28(1):10-8. "TTF-1 expression in ovarian and uterine epithelial neoplasia and its potential significance, an immunohistochemical assessment with multiple monoclonal antibodies and different secondary detection systems." Author(s): Zhang PJ, Gao HG, Pasha TL, Litzky L, Livolsi VA Number of Citations: 2 (See PubMed article )
	Hu / Not Cited	MA5-13972 was used in immunohistochemistry to investigate the role of TTF1 in lung surfactant homeostasis and respiratory system integrity Respir Res. 2011 Aug 25;12():115. "Altered surfactant homeostasis and recurrent respiratory failure secondary to TTF-1 nuclear targeting defect." Author(s): Peca D, Petrini S, Tzialla C, Boldrini R, Morini F, Stronati M, Carnielli VP, Cogo PE, Danhaive O Number of Citations: 1 (See PubMed article )

(This product is for In Vitro experimental use only. Not for resale without express authorization.)