HER-2 Antibody (3B5)

HER-2 Monoclonal Antibody for IHC, IP, Western blot

>> See 51 other antibodies for ERBB2 (c-erb B2/neu protein, herstatin, metastatic lymph node gene 19 protein, neuroblastoma/glioblastoma derived oncogene homolog, p185erbB2, proto-oncogene Neu, proto-oncogene c-ErbB-2, receptor tyrosine-protein kinase erbB-2, tyrosine kinase-type cell surface receptor HER2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog))

Synonyms:

c-erb B2/neu protein, herstatin, metastatic lymph node gene 19 protein, neuroblastoma/glioblastoma derived oncogene homolog, p185erbB2, proto-oncogene Neu, proto-oncogene c-ErbB-2, receptor tyrosine-protein kinase erbB-2, tyrosine kinase-type cell surface receptor HER2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)

Entrez Gene ID:

(Hu) 2064, (Ms) 13866, (Rt) 24337

UniProt ID:

(Hu) P04626, (Ms) P70424

Details

Host / Isotype:	Mouse / IgG1
Class:	Monoclonal
Type:	Antibody
Clone:	3B5
Species Reactivity:	Human (Hu) Mouse (Ms) Rat (Rt) Non-human primate (Nhp)
Immunogen:	A synthetic peptide from the C-terminus of human c-erbB-2 protein. This Sequence Is Identical In Rat Neu Protein.

Ordering Information
Pierce HER-2 Antibody (3B5)

Product #

MA5-13675

Size

500 ul

Price

$308.00

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Storage:	4° C, do not freeze
Form:	500 ul of antibody purified from the ascites fluid by Protein G chromatography (200 ug/ml). Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Applications	Dilution *

Immunohistochemistry (IHC)	1:1000-1:1500
Western Blot (WB)	0.5-1.0 µg/ml
Immunoprecipitation (IP)	2µg/mg protein lysate
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Product Specific Information

MA5-13675 targets HER-2 in IHC, IP, and WB applications and shows reactivity with Human, Murine, Non-human primate, and Rat samples.

The MA5-13675 immunogen is a synthetic peptide from the C-terminus of human c-erbB-2 protein. This Sequence Is Identical In Rat Neu Protein.

General Information

c-erbB-2 is a receptor tyrosine kinase of the c-erbB family. Approximately 25% of primary breast and ovarian tumors were found to overexpress the protein

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HER-2 Antibody (MA5-13675) in IP

Immunoprecipitation with anti-HER-2 Monoclonal Antibody [3B5] (MA5-13675)

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Immunoprecipitation of HER-2 using HER-2 Monoclonal Antibody (MA5-13675) on denatured Human SKBR3 Cells.

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HER-2 Antibody (MA5-13675) in IP

Immunoprecipitation with anti-HER-2 Monoclonal Antibody [3B5] (MA5-13675)

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Immunoprecipitation of HER-2 using HER-2 Monoclonal Antibody (MA5-13675) on Native Human SKBR3 Cells.

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HER-2 Antibody (MA5-13675) in WB

Western Blot with anti-HER-2 Monoclonal Antibody [3B5] (MA5-13675)

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Western blot of HER-2 using HER-2 Monoclonal Antibody (MA5-13675) on SKBR3 Cells and MAD109 Cells.

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PubMed References:

ELISA (1)
FACS (1)
ICC (2)
IHC (10)
IP (1)
WB (4)

ELISA
	Species / Dilution	Summary
	Hu / Not Cited	MA5-13675 was used in ELISA to study the receptor tyrosine kinase activation by through antibody microarrays Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9330-5. "Profiling receptor tyrosine kinase activation by using Ab microarrays." Author(s): Nielsen UB, Cardone MH, Sinskey AJ, MacBeath G, Sorger PK Number of Citations: 1 (See PubMed article )

Flow Cytometry
	Species / Dilution	Summary
	Ms / 0	MA5-13675 was used in flow cytometry and immunohistochemistry to develop a co-culture model from an invasive lobular carcinoma for the evaluation of therapeutic agents BMC Cancer. 2008 Apr 24;8():119. "A stable explant culture of HER2/neu invasive carcinoma supported by alpha-Smooth Muscle Actin expressing stromal cells to evaluate therapeutic agents." Author(s): Piechocki MP Number of Citations: 0 (See PubMed article )

Immunocytochemistry
	Species / Dilution	Summary
	Hu / Not Cited	MA5-13675 was used in immunocytochemistry, immunohistochemistry, and western blot to characterize HER2 in human tumors and cell lines Int J Cancer. 2004 Feb 10;108(4):540-8. "HER-2 receptor expression, localization, and activation in colorectal cancer cell lines and human tumors." Author(s): Half E, Broaddus R, Danenberg KD, Danenberg PV, Ayers GD, Sinicrope FA Number of Citations: 1 (See PubMed article )
	Hu / 1:200	MA5-13675 was used in immunocytochemistry to investigate the mechanism for the malignant rhabdoid tumor treatment with trastuzumab Clin Cancer Res. 2008 Feb 15;14(4):1192-9. "Trastuzumab activates allogeneic or autologous antibody-dependent cellular cytotoxicity against malignant rhabdoid tumor cells and interleukin-2 augments the cytotoxicity." Author(s): Katsumi Y, Kuwahara Y, Tamura S, Kikuchi K, Otabe O, Tsuchiya K, Iehara T, Kuroda H, Hosoi H, Sugimoto T Number of Citations: 1 (See PubMed article )

Immunohistochemistry
	Species / Dilution	Summary
	Av / 1 ug/ml	MA5-13675 was used in immunohistochemistry to investigate the similar changes of p53, ras, and HER-2/neu genes in ovatian adenocarcinomas between chicken and human Cancer Prev Res (Phila). 2009 Feb;2(2):114-21. "Ovarian adenocarcinomas in the laying hen and women share similar alterations in p53, ras, and HER-2/neu." Author(s): Hakim AA, Barry CP, Barnes HJ, Anderson KE, Petitte J, Whitaker R, Lancaster JM, Wenham RM, Carver DK, Turbov J, Berchuck A, Kopelovich L, Rodriguez GC Number of Citations: 1 (See PubMed article )
	Hu / 1:200	MA5-13675 was used in immunocytochemistry, immunohistochemistry, and western blot to characterize HER2 in human tumors and cell lines Int J Cancer. 2004 Feb 10;108(4):540-8. "HER-2 receptor expression, localization, and activation in colorectal cancer cell lines and human tumors." Author(s): Half E, Broaddus R, Danenberg KD, Danenberg PV, Ayers GD, Sinicrope FA Number of Citations: 1 (See PubMed article )
	Hu / 1:2000	MA5-13675 was used in immunohistochemistry to report the clinical cases of cystic hypersecretory carcinoma Histopathology. 2005 Jan;46(1):43-9. "Cystic hypersecretory carcinoma: rare and poorly recognized variant of intraductal carcinoma of the breast. Report of five cases." Author(s): Skalova A, Ryska A, Kajo K, Di Palma S, Kinkor Z, Michal M Number of Citations: 1 (See PubMed article )
	Hu / 1:2000	MA5-13675 was used in immunohistochemistry to characterize non-invasive carcinoma ex pleomorphic adenoma through HER-2/neu and MIB1 expression Histopathology. 2005 Feb;46(2):144-52. "Non-invasive (intracapsular) carcinoma ex pleomorphic adenoma: recognition of focal carcinoma by HER-2/neu and MIB1 immunohistochemistry." Author(s): Di Palma S, Skálová A, Vanìèek T, Simpson RH, Stárek I, Leivo I Number of Citations: 4 (See PubMed article )
	Hu / 1:2000	MA5-13675 was used in immunohistochemistry to investigate clonality of sclerosing polycystic adenosis by human androgen receptor polymorphism Am J Surg Pathol. 2006 Aug;30(8):939-44. "Clonal nature of sclerosing polycystic adenosis of salivary glands demonstrated by using the polymorphism of the human androgen receptor (HUMARA) locus as a marker." Author(s): Skálová A, Gnepp DR, Simpson RH, Lewis JE, Janssen D, Sima R, Vanecek T, Di Palma S, Michal M Number of Citations: 2 (See PubMed article )
	Hu / 0	MA5-13675 was used in immunohistochemistry to study breast cancer survival and tumor characteristics in premenopausal women carrying the CHEK21100delC germline mutation J Clin Oncol. 2007 Jan 1;25(1):64-9. "Breast cancer survival and tumor characteristics in premenopausal women carrying the CHEK21100delC germline mutation."** Author(s): Schmidt MK, Tollenaar RA, de Kemp SR, Broeks A, Cornelisse CJ, Smit VT, Peterse JL, van Leeuwen FE, Van't Veer LJ Number of Citations: 14 (See PubMed article )
	Hu / 1:250	MA5-13675 was used in immunohistochemistry to report on a case of primary neuroendocrine carcinoma of the breast Tumori. 2007 Sep-Oct;93(5):496-8. "Primary neuroendocrine carcinoma of the breast: a case report." Author(s): Yaren A, Kelten C, Akbulut M, Teke Z, Duzcan E, Erdem E Number of Citations: 1 (See PubMed article )
	Hu / 0	MA5-13675 was used in flow cytometry and immunohistochemistry to develop a co-culture model from an invasive lobular carcinoma for the evaluation of therapeutic agents BMC Cancer. 2008 Apr 24;8():119. "A stable explant culture of HER2/neu invasive carcinoma supported by alpha-Smooth Muscle Actin expressing stromal cells to evaluate therapeutic agents." Author(s): Piechocki MP Number of Citations: 0 (See PubMed article )
	Hu / 1:200	MA5-13675 was used in immunohistochemistry to study the prognostic value of stromal caveolin-1 in breast cancer Pathol Oncol Res. 2012 Apr;18(2):459-69. "Stromal caveolin-1 expression in breast carcinoma. Correlation with early tumor recurrence and clinical outcome." Author(s): El-Gendi SM, Mostafa MF, El-Gendi AM Number of Citations: 4 (See PubMed article )
	Ms / 1:70	MA5-13675 was used in immunohistochemistry to study the effect of vitamin D receptor gene dosage on mammary gland morphology and tumorigenesis Carcinogenesis. 2004 Dec;25(12):2361-72. "Vitamin D receptor status alters mammary gland morphology and tumorigenesis in MMTV-neu mice." Author(s): Zinser GM, Welsh J Number of Citations: 8 (See PubMed article )

Immunoprecipitation
	Species / Dilution	Summary
	Hu / Not Cited	MA5-13675 was used in immunoprecipitation to investigate the state of HER1-HER2 interaction and modification in cell lines and tumors Breast Cancer Res. 2011 Apr 15;13(2):R44. "Quantitative assays for the measurement of HER1-HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of action." Author(s): DeFazio-Eli L, Strommen K, Dao-Pick T, Parry G, Goodman L, Winslow J Number of Citations: 1 (See PubMed article )

Western Blot
	Species / Dilution	Summary
	Hu / 1:400	MA5-13675 was used in immunocytochemistry, immunohistochemistry, and western blot to characterize HER2 in human tumors and cell lines Int J Cancer. 2004 Feb 10;108(4):540-8. "HER-2 receptor expression, localization, and activation in colorectal cancer cell lines and human tumors." Author(s): Half E, Broaddus R, Danenberg KD, Danenberg PV, Ayers GD, Sinicrope FA Number of Citations: 1 (See PubMed article )
	Hu / 1:200	MA5-13675 was used in western blot to study the effects of vitamin D receptor agonists on androgen-dependent LNCaP human prostate cancer cell growth J Steroid Biochem Mol Biol. 2005 Oct;97(1-2):37-46. "Vitamin D receptor agonists induce prostatic acid phosphatase to reduce cell growth and HER-2 signaling in LNCaP-derived human prostate cancer cells." Author(s): Stewart LV, Lyles B, Lin MF, Weigel NL Number of Citations: 3 (See PubMed article )
	Hu / 1:400	MA5-13675 was used in western blot to examine the effect of anti-EGFR and ErbB-2 antibodies on the survival of human colon cancer cells Cancer Lett. 2007 Jun 28;251(2):237-46. "Anti-EGFR and ErbB-2 antibodies attenuate cyclooxygenase-2 expression and cooperatively inhibit survival of human colon cancer cells." Author(s): Half E, Sun Y, Sinicrope FA Number of Citations: 1 (See PubMed article )
	Hu / 0	MA5-13675 was used in western blot to investigate the impact of epidermal growth factor on neuroendocrine differentiation of LNCaP cells Neoplasia. 2007 Aug;9(8):614-24. "EGF prevents the neuroendocrine differentiation of LNCaP cells induced by serum deprivation: the modulator role of PI3K/Akt." Author(s): Martín-Orozco RM, Almaraz-Pro C, Rodríguez-Ubreva FJ, Cortés MA, Ropero S, Colomer R, López-Ruiz P, Colás B Number of Citations: 7 (See PubMed article )

(This product is for In Vitro experimental use only. Not for resale without express authorization.)