XRCC1 Antibody, Biotin conjugate (33-2-5)

XRCC1 Monoclonal Antibody for IHC (P), IP, Western blot

>> See 4 other antibodies for XRCC1 (DNA repair protein XRCC1, X-ray repair cross-complementing protein 1, X-ray-repair, complementing defective, repair in Chinese hamster)

Synonyms:

DNA repair protein XRCC1, X-ray repair cross-complementing protein 1, X-ray-repair, complementing defective, repair in Chinese hamster

Entrez Gene ID:

(Hu) 7515, (Rt) 84495

UniProt ID:

(Hu) P18887, (Rt) Q9ESZ0

Details

Host / Isotype:	Mouse / IgG2b
Class:	Monoclonal
Type:	Antibody
Clone:	33-2-5
Label:	Biotin
Species Reactivity:	Human (Hu) Rat (Rt)
Immunogen:	Recombinant human XRCC1 protein

Ordering Information
Pierce XRCC1 Antibody, Biotin conjugate (33-2-5)

Product #

MA5-13409

Size

500 ul

Price

$420.00

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Storage:	4° C, do not freeze
Form:	500 ul of antibody purified from ascites fluid by Protein A chromatography (200 ug/ml). Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Applications	Dilution *

Immunohistochemistry (Paraffin) (IHC (P))	1-2 µg/ml
Immunoprecipitation (IP)	2 µg/ml
Western Blot (WB)	1-2 µg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Product Specific Information

MA5-13409 targets XRCC1 in IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples.

The MA5-13409 immunogen is recombinant human XRCC1 protein.

General Information

X-Ray Repair Cross Complementing (XRCC1) plays a role in excision repair of DNA after ionizing irradiation. XRCC1 binds to DNA ligase III through the C-terminal 96 amino acids and to DNA polymerase beta through the N-terminal half. In testis XRCC1 is expressed at high levels. Cells with a mutation of this gene exhibit decreased single strand break repair and reduced recombination repair. They show increased double strand breaks, and sister chromatid exchange is increased up to 10-fold. The XRCC1 might serve as a scaffold protein during base excision-repair.

Product Images

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XRCC1 Antibody (MA5-13409) in IHC

Immunohistochemistry with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409)

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Formalin-fixed, paraffin-embedded human testis stained with XRCC1 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of spermatocytes.

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XRCC1 Antibody (MA5-13409) in IHC

Immunohistochemistry with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409)

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XRCC1 Antibody (MA5-13409) in IHC

Immunohistochemistry with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409)

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Formalin-fixed, paraffin-embedded rat testis stained with XRCC1 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of spermatocytes.

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XRCC1 Antibody (MA5-13409) in IP

Immunoprecipitation with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409)

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Immunoprecipitation of XRCC1 using XRCC1 Monoclonal Antibody (MA5-13409) on Native Human LS174T Cells.

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XRCC1 Antibody (MA5-13409) in WB

Western Blot with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409)

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Western blot of XRCC1 using XRCC1 Monoclonal Antibody (MA5-13409) on LS174T Cells.

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PubMed References:

ICC (3)
IHC (1)
WB (4)

Immunocytochemistry
	Species / Dilution	Summary
	Hu / 1:200	MA5-13409 was used in immunocytochemistry and western blot to investigate the influence of XRCC1 on camptothecin resistance Cancer Res. 2002 Jan 15;62(2):459-65. "X-ray repair cross-complementing gene I protein plays an important role in camptothecin resistance." Author(s): Park SY, Lam W, Cheng YC Number of Citations: 5 (See PubMed article )
	Hu / 1:50	MA5-13409 was used in immunocytochemistry to study the mammalian repair mechanism for oxidation-induced DNA damage Proc Natl Acad Sci U S A. 2004 Sep 21;101(38):13738-43. "In situ analysis of repair processes for oxidative DNA damage in mammalian cells." Author(s): Lan L, Nakajima S, Oohata Y, Takao M, Okano S, Masutani M, Wilson SH, Yasui A Number of Citations: 58 (See PubMed article )
	Hu / 1:500	MA5-13409 was used in immunocytochemistry and western blot to investigate the effect of XRCC1(R280H) variant protein on DNA repair Mutat Res. 2005 Apr 4;582(1-2):135-45. "The Arg280His polymorphism in X-ray repair cross-complementing gene 1 impairs DNA repair ability." Author(s): Takanami T, Nakamura J, Kubota Y, Horiuchi S Number of Citations: 1 (See PubMed article )

Immunohistochemistry
	Species / Dilution	Summary
	Hu / 1:300	MA5-13409 was used in immunohistochemistry to investigate the usefulness of specific gene expression in non-small cell lung cancer treatment prognosis Lung Cancer. 2010 Jun;68(3):478-83. "The prognostic significance of ERCC1, BRCA1, XRCC1, and betaIII-tubulin expression in patients with non-small cell lung cancer treated by platinum- and taxane-based neoadjuvant chemotherapy and surgical resection." Author(s): Kang CH, Jang BG, Kim DW, Chung DH, Kim YT, Jheon S, Sung SW, Kim JH Number of Citations: 1 (See PubMed article )

Western Blot
	Species / Dilution	Summary
	Hu / 0	MA5-13409 was used in immunocytochemistry and western blot to investigate the influence of XRCC1 on camptothecin resistance Cancer Res. 2002 Jan 15;62(2):459-65. "X-ray repair cross-complementing gene I protein plays an important role in camptothecin resistance." Author(s): Park SY, Lam W, Cheng YC Number of Citations: 5 (See PubMed article )
	Hu / 1:500	MA5-13409 was used in immunocytochemistry and western blot to investigate the effect of XRCC1(R280H) variant protein on DNA repair Mutat Res. 2005 Apr 4;582(1-2):135-45. "The Arg280His polymorphism in X-ray repair cross-complementing gene 1 impairs DNA repair ability." Author(s): Takanami T, Nakamura J, Kubota Y, Horiuchi S Number of Citations: 1 (See PubMed article )
	Hu / 1:150	MA5-13409 was used in western blot to investigate the relation between XRCC1 expression and apoptosis in breast cancer cell lines Breast Cancer Res Treat. 2006 Oct;99(3):257-65. "An intronic polymorphism associated with increased XRCC1 expression, reduced apoptosis and familial breast cancer." Author(s): Bu D, Tomlinson G, Lewis CM, Zhang C, Kildebeck E, Euhus DM Number of Citations: 1 (See PubMed article )
	Hu / 0	MA5-13409 was used in western blot to study the role of Chk2-dependent XRCC1 phosphorylation in the DNA damage response EMBO J. 2008 Dec 3;27(23):3140-50. "Chk2-dependent phosphorylation of XRCC1 in the DNA damage response promotes base excision repair." Author(s): Chou WC, Wang HC, Wong FH, Ding SL, Wu PE, Shieh SY, Shen CY Number of Citations: 7 (See PubMed article )

(This product is for In Vitro experimental use only. Not for resale without express authorization.)