Cyclin A Antibody (6E6)

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Cyclin A Monoclonal Antibody for IHC (P), IP, Western blot

>> See 1 other antibody for CCNA1 (cyclin-A1)

Synonyms:

cyclin-A1

Entrez Gene ID:

(Hu) 8900

UniProt ID:

(Hu) P78396

Details

Host / Isotype:	Mouse / IgG1, kappa
Class:	Monoclonal
Type:	Antibody
Clone:	6E6
Species Reactivity:	Human (Hu)
Immunogen:	Recombinant N-terminal fragment of human cyclin A protein

Ordering Information
Pierce Cyclin A Antibody (6E6)

Product #

MA5-11306

Size

500 ul

Price

$308.00

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Storage:	4° C
Form:	500 ul of tissue culture supernatant with 0.09% sodium azide.

Applications	Dilution *

Immunohistochemistry (Paraffin) (IHC (P))	Assay Dependent
Immunoprecipitation (IP)	Assay Dependent
Western Blot (WB)	1:100
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Product Specific Information

MA5-11306 targets Cyclin A in IHC (P), IP, and WB applications and shows reactivity with Human samples.

The MA5-11306 immunogen is recombinant N-terminal fragment of human cyclin A protein.

General Information

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's). They are expressed temporally to control transition at different specific phases of the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase.

Product Images

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Cyclin A Antibody (MA5-11306) in IHC

Immunohistochemistry with anti-Cyclin A Monoclonal Antibody [6E6] (MA5-11306)

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Formalin-fixed, paraffin-embedded human tonsil stained with Cyclin A antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of proliferating cells.

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Cyclin A Antibody (MA5-11306) in IP

Immunoprecipitation with anti-Cyclin A Monoclonal Antibody [6E6] (MA5-11306)

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Immunoprecipitation of Cyclin A using Cyclin A Monoclonal Antibody (MA5-11306) on Native Human HeLa Cells.

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Cyclin A Antibody (MA5-11306) in WB

Western Blot with anti-Cyclin A Monoclonal Antibody [6E6] (MA5-11306)

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Western blot of Cyclin A using Cyclin A Monoclonal Antibody (MA5-11306) on HeLa Cells.

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PubMed References:

IHC (1)
WB (4)

Immunohistochemistry
	Species / Dilution	Summary
	Hu / 1:20	MA5-11306 was used in immunohistochemistry to study the utility of human EGF 2 and cyclin A2 in predicting recurrence of invasive breast cancer Chin Med J (Engl). 2010 Feb 20;123(4):431-7. "Prediction of recurrence risk in early breast cancer using human epidermal growth factor 2 and cyclin A2." Author(s): Li HP, Ji JF, Hou KY, Lei YT, Zhao HM, Wang J, Zheng J, Liu JY, Wang MP, Xiao Y, Wang YF, Lü YY, Sun Y Number of Citations: 1 (See PubMed article )

Western Blot
	Species / Dilution	Summary
	Hu / 0	MA5-11306 was used in western blot and western blot to investigate the effect of HCV NS2 protein on cell proliferation and cell cycle and its mechanism Virus Res. 2006 Nov;121(2):134-43. "HCV NS2 protein inhibits cell proliferation and induces cell cycle arrest in the S-phase in mammalian cells through down-regulation of cyclin A expression." Author(s): Yang XJ, Liu J, Ye L, Liao QJ, Wu JG, Gao JR, She YL, Wu ZH, Ye LB Number of Citations: 1 (See PubMed article )
	Hu / 0	MA5-11306 was used in western blot to investigate a novel adenoviral method of gene therapy against human colon carcinoma xenografts Cancer Res. 2007 May 15;67(10):4949-55. "Suicide gene therapy of human colon carcinoma xenografts using an armed oncolytic adenovirus expressing carboxypeptidase G2." Author(s): Schepelmann S, Ogilvie LM, Hedley D, Friedlos F, Martin J, Scanlon I, Chen P, Marais R, Springer CJ Number of Citations: 1 (See PubMed article )
	Hu / 0	MA5-11306 was used in western blot to study novel human Cdt1-binding proteins using a proteomics approach Mol Biol Cell. 2008 Mar;19(3):1007-21. "Identification of novel human Cdt1-binding proteins by a proteomics approach: proteolytic regulation by APC/CCdh1." Author(s): Sugimoto N, Kitabayashi I, Osano S, Tatsumi Y, Yugawa T, Narisawa-Saito M, Matsukage A, Kiyono T, Fujita M Number of Citations: 14 (See PubMed article )
	Nhp / 0	MA5-11306 was used in western blot and western blot to investigate the effect of HCV NS2 protein on cell proliferation and cell cycle and its mechanism Virus Res. 2006 Nov;121(2):134-43. "HCV NS2 protein inhibits cell proliferation and induces cell cycle arrest in the S-phase in mammalian cells through down-regulation of cyclin A expression." Author(s): Yang XJ, Liu J, Ye L, Liao QJ, Wu JG, Gao JR, She YL, Wu ZH, Ye LB Number of Citations: 1 (See PubMed article )

(This product is for In Vitro experimental use only. Not for resale without express authorization.)