GTPase Activating Protein Antibody (B4F8)
GTPase Activating Protein Monoclonal Antibody for IF, IP, Western blot
Synonyms:
GAP
Entrez Gene ID:
UniProt ID:
(Hu) P20936, (Rt) P50904
| Details | |
| Host / Isotype: | Mouse / IgG2a |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | B4F8 |
| Species Reactivity: | Human (Hu) Mouse (Ms) Rat (Rt) Non-human primate (Nhp) Bovine (Bv) |
| Immunogen: | Full length recombinant human GAP. |
| Ordering Information | ||||
| Pierce GTPase Activating Protein Antibody (B4F8) |
| Storage: | -20° C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µg of lyopholized, protein A purified anti-GAP. Reconstitute with PBS. |
| Applications | Dilution * |
| Western Blot (WB) | 10 µg/ml |
| Immunoprecipitation (IP) | 1-5 µg |
| Immunofluorescence (IF) | 5 µg/ml |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| MA4-001 detects GTPase activating protein (GAP) from human, mouse, rat, bovine and non-human primate tissues. MA4-001 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes a single 120 kDa protein representing ras GAP from RS-2 cell lysate. Immunofluorescence staining of GAP in mouse fibroblast cells with MA4-001 results in diffuse cytoplasmic staining. Following the addition of PDGF, immunofluorescence staining shows that some GAP rapidly translocates to the plasma membrane. MA4-001 immunoprecipitates GAP that is complexed with the GAP related p190. The MA4-001 antigen is full length recombinant human GAP. Epitope mapping studies suggest that this antibody binds a portion of GAP that contains the src homology regions SH2 & SH3. |
| General Information |
| GTPase Activating Protein (GAP) is capable of stimulating the hydrolysis of GTP by ras p21 proteins, though GAP has little effect on the oncogenic forms of ras. It is also known that several tyrosine kinases such as platelet derived growth factor receptor and epidermal growth factor receptor are involved in the tyrosine phosphorylation of GAP. It has therefore been suggested that GAP may provide a connection or link between growth factor receptors and the ras p21 family. |
| PubMed References: |
| Immunoprecipitation | ||
| Species / Dilution | Summary | |
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Hu / Not Cited |
MA4-001 was used in immunoprecipitation and western blot to clone the GAP-associated protein p190 and to investigate its functional properties
Cell. 1992 May 1;69(3):539-49.
"Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications for a signaling pathway from ras to the nucleus." Author(s): Settleman J, Narasimhan V, Foster LC, Weinberg RA Number of Citations: 75 (See PubMed article  )
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| Western Blot | ||
| Species / Dilution | Summary | |
|
Hu / Not Cited |
MA4-001 was used in immunoprecipitation and western blot to clone the GAP-associated protein p190 and to investigate its functional properties
Cell. 1992 May 1;69(3):539-49.
"Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications for a signaling pathway from ras to the nucleus." Author(s): Settleman J, Narasimhan V, Foster LC, Weinberg RA Number of Citations: 75 (See PubMed article )
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(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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GTPase Activating Protein Antibodies > GTPase Activating Protein Monoclonal Antibody MA4-001
