Lamin A/C Antibody (mab636)

Lamin A/C Monoclonal Antibody for IF, IHC (F), Western blot

>> See 13 other antibodies for LMNA (70 kDa lamin)
Synonyms:
70 kDa lamin
Entrez Gene ID:
(Hu) 4000
UniProt ID:
(Hu) P02545
Details
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Clone: mab636
Species Reactivity: Human (Hu) Bovine (Bv) Porcine (Po)
Immunogen: Porcine lamin preparation.
Ordering Information
Pierce Lamin A/C Antibody (mab636)
Product #
MA3-1000
Size
200 µl
Price
$199.00
Purchase
Add Lamin A/C Antibody (mab636) to your cart.
Storage: -20° C, Avoid Freeze/Thaw Cycles
Form: 200 µl of IgG2b tissue culture supernatant containing 0.05% sodium azide.


Applications Dilution *
Immunohistochemistry (Frozen) (IHC (F)) 1:100
Western Blot (WB) 1:100 - 1:2000
Immunofluorescence (IF) 1:100
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA3-1000 detects lamin A/C from human, bovine and porcine samples.

MA3-1000 has been successfully used in Western blot, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects ~ 70 and 65 kDa proteins corresponding to lamin A and C, respectively, from HeLa cell extract. This antibody can also be used for immunofluorescence and immunohistochemistry for subcellular localization experiments. MA3-1000 has been widely used for detection of lamin A/C control in siRNA experiments.

The MA3-1000 antigen is porcine lamin preparation.
General Information
Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).
Product Images
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  • Lamin A/C Antibody (MA3-1000) in IF
    Immunofluorescence with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    Immunofluorescence with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    View Product Details >>
    Immunofluorescent analysis of Lamin A/C using anti-Lamin A/C monoclonal antibody (Product# MA3-1000) shows staining in A549 Cells.
     
  • Lamin A/C Antibody (MA3-1000) in IF
    Immunofluorescence with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    Immunofluorescence with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    View Product Details >>
    Immunofluorescent analysis of Lamin A/C using Lamin A/C Monoclonal Antibody (mab636) (Product# MA3-1000 ) shows staining in Hela Cells. Lamin A/C (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C (Product# MA3-1000 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
     
  • Lamin A/C Antibody (MA3-1000) in IF
    Immunofluorescence with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    Immunofluorescence with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    View Product Details >>
    Immunofluorescent analysis of Lamin A/C using anti-Lamin A/C monoclonal antibody (Product# MA3-1000) shows staining in HMVEC Cells.
     
  • Lamin A/C Antibody (MA3-1000) in IF
    Immunofluorescence with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    Immunofluorescence with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    View Product Details >>
    Immunofluorescent analysis of Lamin A/C using Lamin A/C Monoclonal Antibody (mab636) (Product# MA3-1000 ) shows staining in U251 Cells. Lamin A/C (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C (Product# MA3-1000 ) at a dilution of 1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
     
  • Lamin A/C Antibody (MA3-1000) in IF
    Immunofluorescence with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    Immunofluorescence with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    View Product Details >>
    Immunofluorescent analysis of Lamin A/C (red) in HeLa cells at high magnification.
     
  • Lamin A/C Antibody (MA3-1000) in IF
    Immunofluorescence with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    Immunofluorescence with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    View Product Details >>
    Immunofluorescent analysis of Lamin A/C (green) in untreated U2-OS cells (A) or HeLa cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 0.3% BSA for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C (Product # MA3-1000), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product# 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
     
  • Lamin A/C Antibody (MA3-1000) in IF
    Immunofluorescence with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    Immunofluorescence with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    View Product Details >>
    Immunofluorescent analysis of Lamin A/C (green) in Human fibroblast cells. Methanol fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C (Product# MA3-1000), at a dilution of 1:200 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature.
     
  • Lamin A/C Antibody (MA3-1000) in WB
    Western Blot with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    Western Blot with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    View Product Details >>
    Western blot analysis of Lamin A/C was performed by loading various amounts of Human fibroblast whole cell lysate onto a 10% SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with PBS-0.002% Tween-20 containing 3% non-fat dry milk for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing Lamin A/C (Product #MA3-1000) at a dilution of 1:2000 overnight at 4ºC on a rocking platform. Membranes were then washed in PBS-0.002% Tween-20 and probed with a goat anti-mouse-HRP secondary antibody (Product #32430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Pico (Product # 37087).
     
  • Lamin A/C Antibody (MA3-1000) in WB
    Western Blot with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    Western Blot with anti-Lamin A/C Monoclonal Antibody [mab636] (MA3-1000)

    View Product Details >>
    Western blot analysis of Lamin A/C was performed by loading 10µg nuclear extract from fractionated mouse tissue lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing phospho-Lamin A/C (Product #MA3-1000) at a dilution of 1:500 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:15000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura (Product #34075).
     
 
 
PubMed References:
Immunocytochemistry
  Species / Dilution Summary
  Hu / Not Cited
Ms / Not Cited
 MA3-1000 was used in immunocytochemistry and western blot to investigate the effect of 21nt siRNA duplexes for RNAi in mammalian cells
Nature. 2001 May 24;411(6836):494-8.
"Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells."
Author(s): Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
 MA3-1000 was used in western blot and immunocytochemistry to study the effect of thioglycollate on the expression of nuclear lamins A/C
Exp Cell Res. 1990 Oct;190(2):185-94.
"Induction of nuclear lamins A/C in macrophages in in vitro cultures of rat bone marrow precursor cells and human blood monocytes, and in macrophages elicited in vivo by thioglycollate stimulation."
Author(s): Röber RA, Gieseler RK, Peters JH, Weber K, Osborn M
Number of Citations: 3
(See PubMed article )
Western Blot
  Species / Dilution Summary
  Hu / Not Cited
 MA3-1000 was used in western blot and immunocytochemistry to study the effect of thioglycollate on the expression of nuclear lamins A/C
Exp Cell Res. 1990 Oct;190(2):185-94.
"Induction of nuclear lamins A/C in macrophages in in vitro cultures of rat bone marrow precursor cells and human blood monocytes, and in macrophages elicited in vivo by thioglycollate stimulation."
Author(s): Röber RA, Gieseler RK, Peters JH, Weber K, Osborn M
Number of Citations: 3
(See PubMed article )
  Hu / 1:100
 MA3-1000 was used in immunocytochemistry and western blot to investigate the effect of 21nt siRNA duplexes for RNAi in mammalian cells
Nature. 2001 May 24;411(6836):494-8.
"Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells."
Author(s): Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T
Number of Citations: 1
(See PubMed article )
  Hu / 1:200
 MA3-1000 was used in western blot to investigate the effect of RANBP 16 and 17 on bHLH transcription factor E12 function
J Cell Biochem. 2010 Sep 1;111(1):195-206.
"Identification of RANBP16 and RANBP17 as novel interaction partners for the bHLH transcription factor E12."
Author(s): Lee JH, Zhou S, Smas CM
Number of Citations: 1
(See PubMed article )
  Ms / 1:100
 MA3-1000 was used in immunocytochemistry and western blot to investigate the effect of 21nt siRNA duplexes for RNAi in mammalian cells
Nature. 2001 May 24;411(6836):494-8.
"Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells."
Author(s): Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T
Number of Citations: 1
(See PubMed article )
  Po / Not Cited
 MA3-1000 was used in western blot and immunocytochemistry to study the effect of thioglycollate on the expression of nuclear lamins A/C
Exp Cell Res. 1990 Oct;190(2):185-94.
"Induction of nuclear lamins A/C in macrophages in in vitro cultures of rat bone marrow precursor cells and human blood monocytes, and in macrophages elicited in vivo by thioglycollate stimulation."
Author(s): Röber RA, Gieseler RK, Peters JH, Weber K, Osborn M
Number of Citations: 3
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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