AP2 Antibody (3B5)

AP2 Monoclonal Antibody for IF, IHC, IP, Western blot

>> See 7 other antibodies for TFAP2A (ERF-1)
Synonyms:
ERF-1
Entrez Gene ID:
(Hu) 7020, (Ms) 21418
UniProt ID:
(Hu) P05549, (Ms) P34056
Details
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Clone: 3B5
Species Reactivity: Human (Hu) Mouse (Ms) Chicken (Ck)
Immunogen: N-terminus of human AP2a.
Ordering Information
Pierce AP2 Antibody (3B5)
Product #
MA1-872
Size
100 µl
Price
$299.00
Purchase
Add AP2 Antibody (3B5) to your cart.
Storage: -20° C, Avoid Freeze/Thaw Cycles
Form: 100 µl of protein G purified mouse IgG2b in PBS with 1 mg/ml BSA and 0.05% sodium azide.


Applications Dilution *
Immunoprecipitation (IP) 1:100 - 1:250
Immunofluorescence (IF) 1:50 - 1:500
Immunohistochemistry (IHC) 1:50
Western Blot (WB) 1:100 - 1:1,000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA1-872 detects AP-2 from human, mouse, and chicken samples.

MA1-872 has been successfully used in Western blot, immunoprecipitation, and immunofluorescence applications. By Western blot, this antibody detects a 45-50 kDa band representing AP-2. For Western blotting, block with BSA only (not milk).

The MA1-872 immunogen is the N-terminus of human AP2a.
General Information
AP-2, also known as ERF-1, plays an important role in regulating estrogen receptor expression. It also may play a role in the development of ectodermal-derived tissue. Mutations in the AP-2 gene have been linked to many eye chamber defects.
Product Images
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  • AP2 Antibody (MA1-872) in IF
    Immunofluorescence with anti-AP2 Monoclonal Antibody [3B5] (MA1-872)

    Immunofluorescence with anti-AP2 Monoclonal Antibody [3B5] (MA1-872)

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    Immunofluorescent analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) (Product# MA1-872) shows staining in Hela Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing AP2 (Product# MA1-872) at a dilution of 1:100 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
     
  • AP2 Antibody (MA1-872) in IF
    Immunofluorescence with anti-AP2 Monoclonal Antibody [3B5] (MA1-872)

    Immunofluorescence with anti-AP2 Monoclonal Antibody [3B5] (MA1-872)

    View Product Details >>
    Immunofluorescent analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) (Product# MA1-872) shows staining in MCF-7 Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing AP2 (Product# MA1-872) at a dilution of 1:20 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
     
  • AP2 Antibody (MA1-872) in IF
    Immunofluorescence with anti-AP2 Monoclonal Antibody [3B5] (MA1-872)

    Immunofluorescence with anti-AP2 Monoclonal Antibody [3B5] (MA1-872)

    View Product Details >>
    Immunofluorescent analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) (Product# MA1-872) shows staining in U251 Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing AP2 (Product# MA1-872) at a dilution of 1:100 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
     
  • AP2 Antibody (MA1-872) in IHC
    Immunohistochemistry with anti-AP2 Monoclonal Antibody [3B5] (MA1-872)

    Immunohistochemistry with anti-AP2 Monoclonal Antibody [3B5] (MA1-872)

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    Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing AP2 (MA1-872) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
     
  • AP2 Antibody (MA1-872) in WB
    Western Blot with anti-AP2 Monoclonal Antibody [3B5] (MA1-872)

    Western Blot with anti-AP2 Monoclonal Antibody [3B5] (MA1-872)

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    Western blot detection of AP-2 from MCF7 cell lysate.
     
 
 
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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