C/EBP alpha Antibody (15C8)

Printable Datasheet

View 1 Product Image

View 4 References

C/EBP alpha Monoclonal Antibody for IP, Western blot

>> See 6 other antibodies for CEBPA (CCAAT/Enhancer Binding Protein Alpha)

Synonyms:

CCAAT/Enhancer Binding Protein Alpha

Entrez Gene ID:

(Hu) 1050, (Ms) 12606, (Rt) 24252

UniProt ID:

(Hu) P49715, (Ms) P53566, (Rt) P05554

Details

Host / Isotype:	Mouse / IgG
Class:	Monoclonal
Type:	Antibody
Clone:	15C8
Species Reactivity:	Human (Hu) Mouse (Ms) Rat (Rt)
Immunogen:	Synthetic peptide corresponding to residues M(1) E S A D F Y E V E P R P P(14) of mouse C/EBP alpha.

Ordering Information
Pierce C/EBP alpha Antibody (15C8)

Product #

MA1-825

Size

100 µg

Price

$299.00

Purchase

Bulk Order?

Storage:	-20° C, Avoid Freeze/Thaw Cycles
Form:	100 ug of protein G purified IgG (1mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide.

Applications	Dilution *

Western Blot (WB)	2 ug/ml
Immunoprecipitation (IP)	Assay dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Product Specific Information

MA1-825 detects C/EBP alpha protein in human, mouse and rat samples.

MA1-825 has successfully been used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 45 kDa protein representing C/EBP alpha in transfected hu293T cells. MA1-825 is specific to the amino terminal region of C/EBP alpha.

The MA1-825 immunogen is a synthetic peptide corresponding to residues M(1) E S A D F Y E V E P R P P(14) of mouse C/EBP alpha. This peptide (Cat. # PEP-242) is available for use in neutralization and control experiments.

General Information

The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues. These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP. One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells. Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation. From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients.

Product Images

Filter :

C/EBP alpha Antibody (MA1-825) in WB

Western Blot with anti-C/EBP alpha Monoclonal Antibody [15C8] (MA1-825)

View Product Details >>

Western blot detection of C/EBP alpha on transfected 293T cells using MA1-825.

... more >>

PubMed References:

ICC (1)
IP (1)
WB (2)

Immunocytochemistry
	Species / Dilution	Summary
	Hu / Not Cited	MA1-825 was used in immunocytochemistry to investigate the role of C/EBP-alpha on the regulation of rRNA synthesis and cell growth and proliferation EMBO J. 2010 Mar 3;29(5):897-909. "Nucleolar retention of a translational C/EBPalpha isoform stimulates rDNA transcription and cell size." Author(s): Müller C, Bremer A, Schreiber S, Eichwald S, Calkhoven CF Number of Citations: 1 (See PubMed article )

Immunoprecipitation
	Species / Dilution	Summary
	Rt / Not Cited	MA1-825 was used in immunoprecipitation and western blot to investigate the interactions of C/EBPalpha or C/EBPalpha with NF-kappaB p50 and their effect on apoptosis Leukemia. 2009 Feb;23(2):365-74. "C/EBPalpha or C/EBPalpha oncoproteins regulate the intrinsic and extrinsic apoptotic pathways by direct interaction with NF-kappaB p50 bound to the bcl-2 and FLIP gene promoters." Author(s): Paz-Priel I, Ghosal AK, Kowalski J, Friedman AD Number of Citations: 1 (See PubMed article )

Western Blot
	Species / Dilution	Summary
	Ms / Not Cited	MA1-825 was used in western blot to study the interaction between C/EBPalpha and nuclear factor-kappaB p50 Mol Cancer Res. 2005 Oct;3(10):585-96. "CCAAT/enhancer binding protein alpha (C/EBPalpha) and C/EBPalpha myeloid oncoproteins induce bcl-2 via interaction of their basic regions with nuclear factor-kappaB p50." Author(s): Paz-Priel I, Cai DH, Wang D, Kowalski J, Blackford A, Liu H, Heckman CA, Gombart AF, Koeffler HP, Boxer LM, Friedman AD Number of Citations: 1 (See PubMed article )
	Rt / Not Cited	MA1-825 was used in immunoprecipitation and western blot to investigate the interactions of C/EBPalpha or C/EBPalpha with NF-kappaB p50 and their effect on apoptosis Leukemia. 2009 Feb;23(2):365-74. "C/EBPalpha or C/EBPalpha oncoproteins regulate the intrinsic and extrinsic apoptotic pathways by direct interaction with NF-kappaB p50 bound to the bcl-2 and FLIP gene promoters." Author(s): Paz-Priel I, Ghosal AK, Kowalski J, Friedman AD Number of Citations: 1 (See PubMed article )

(This product is for In Vitro experimental use only. Not for resale without express authorization.)